关键词: 细胞培养, 方法, 视网膜, 细胞学

Abstract: Objective To investigate the developmental changes of neonatal bovine retinal horizontal cells in vitro. Methods Dissociated neonatal bovine retinal cells were cultured. Cells grown for 10, 20, 30 and 40 days in vitro were used for immunocytochemical research. Retinal horizontal cells were identified by Calbindin-D28K antibody and the immunoreactivity of stained cells was measured by image analysis system. Results The retinal horizontal cells grown for 20 days in vitro had mature morphological characteristics. The morphology of neurons grown for 30 days and 40 days in vitro was same as that of 20 days in vitro. The immunoreactivity of the stained cells cultured for 20 days in vitro trended to increase when compared with that of 10 days in vitro. The immunoreactivity of cells cultured for 30 days and 40 days in vitro decreased significantly when compared with that of 10 days and 20 days in vitro resp ectively. Conclusion The horizontal cells cultured for 20 days in vitro have mature morphological characteristics and stronger immunoreactivity. They are well developed neurons in vitro.

Key words: cell culture, methods, ret ina, cytology