关键词: 缓释纤维毡, 抑制率, 肝肿瘤, 细胞凋亡

Abstract: To investigate the anti-tumor activity of ADM-fiber on mo use transplanted hepatoma. Methods The ADM-fiber was implanted into H22-bearing C57BL mice. They were divided into four groups: experimental group (ADM-fiber group ), control 1 group (ADM intratumorally injection), control 2 group(ADM intravenous injection), control 3 group(physiologic saline intravenous injection). The tumor volume was calculated on the 1st,3rd,6th,9th,13th day after administration. The tumors were dissected on the 14th day. This biopsy was weighted and the tumor inhibitory rate was then calculated as formula. Biopsy was then observed with HE staining, analyzed with immunohistochemistry method on the expression of apoptosis relative proteins caspase-3/fas, and the apoptotic rate was analyzed using flow cytometer. Results The growth of tumor volume was slower in experimental group and control 1 group on the 6th day than those in other groups (P<0.05), it was slower in experimental group than those in the control 1 group on the 9th,13th day, and it was obviously slower than those in the control 2 and 3 groups on the 9th and 13th day;the inhibitory rate of tumor growth of experinental group was significantly higher than those in the others（P<0.01）; the necrosis of tumor in experimental group was serious than other groups, and the expression of caspase-3/fas was increased numerously in the experimental group(P<0.01); compared with control groups,the apoptotic rate was also enhanced substantially in experimental group(P<0.01）. Conclusion ADM-fiber embedded can exhibit significantly tumor inhibition on mouse H22 transplanted hepatoma, which illustrates that embedding retarders can significantly accelerate the apoptotic rate of tumor cells through ADM-fiber system.

Key words: delayed-release fiber system, inhibitory rate, liver neoplasms, apoptosis