关键词: 一氧化氮, 肝硬化, 胆汁性, 肠道屏障

Abstract: To explore the effect of glycine on the intestinal barrier of rats with obstructive jaundice. Methods The male Wistar rats weighing 220—260g were randomly assigned to three groups(n=20): sham operation group (A), common bile ligation gruop(B), common bile duct ligation plus glycine group(C). Except sham operation group (A), rat models with obstructive jaundice in the other groups were set up by double ligation of the common bile duct. The rats in group C was fed with 5% glycine solution for 5 d. From the second day after the operation , the rats in group C were fed according to the preoperative standard. On the 21th day after operation, the rats were killed and serum bilirubin (BIL) and the amount of nitric oxide (NO) in intestinal tissue were determined, the morphological changes of the intestine were observed, the villus height and mucosal thickness were measured. Results Compared with group A, the serum BIL and the amount of NO in intestinal tissue were increased and the villus height and mucosal thickness were reduced in group B (P<0.01). As compared with group B, the amount of NO were reduced in group C (P<0.01), the architecture distortion in the intestinal tissue was alleviated, an increase was noted in the villus height and mucosal thickness (P<0.01). In group C, the BIL increased (P<0.01), however there were no significant differences of the amount of NO, villus height, mucosal thickness (P>0.05) compared with group A. Conclusion Glycine can decrease the level of NO in the intestinal tissue and improve the damaged intestinal barrier obviously.

Key words: nitric oxide, liver cirrhosis, biliary , intestinal barrier