吉林大学学报(医学版)

• 基础研究 • 上一篇    下一篇

血必净对LPS诱导血管内皮细胞NO产生和iNOS及核转录因子κB蛋白表达的影响

宋丽娟,韩 彬   

  1. 辽宁医学院附属第一医院呼吸感染科,辽宁 锦州 121001
  • 收稿日期:2013-11-28 出版日期:2014-09-28 发布日期:2014-11-24
  • 通讯作者: 韩 彬(Tel:0416-4197095, E-mail:hanbin9500@163.com) E-mail:hanbin9500@163.com
  • 作者简介:宋丽娟(1981-),女,山西省大同市人,在读医学硕士,主要从事呼吸感染与脓毒症方面的研究。
  • 基金资助:

    辽宁省科技厅自然科学基金资助课题 (20092189)

Influence of Xuebijing in production of NO and  expressions of iNOS and NF-κB induced by LPS in vascular endothelial cells

SONG Li-juan,HAN Bin   

  • Received:2013-11-28 Online:2014-09-28 Published:2014-11-24

摘要:

目的:探讨血必净对脂多糖( LPS )诱导血管内皮细胞(VEC)损伤的保护作用,研究在血必净干预下一氧化氮(NO)产生、诱导型一氧化氮合酶(iNOS)表达及信号转导机制。方法:将体外培养的VEC分为对照组、LPS(1 mg/L) 组、LPS(1 mg/L)+血必净( 25 g/L) 组、LPS( 1 mg/L )+吡咯烷二硫代氨基甲酸盐( PDTC, 20 μmol/L ) 组,在给予LPS前预先用血必净和PDTC孵育1 h。采用Western blotting法检测iNOS和核转录因子-κB p65 (NF-κB p65)蛋白的表达情况,采用Griess法检测上清液中NO的水平。结果:与对照组比较,LPS组VEC中NO水平、iNOS和NF-κB p65蛋白表达水平明显升高(P<0.01)。与LPS组比较,LPS+血必净组VEC中NO水平、iNOS和NF-κB p65蛋白表达水平明显降低(P<0.05或P<0.01);与LPS组比较,LPS+PDTC组VEC中NO水平和iNOS及NF-κB p65蛋白表达水平均明显降低(P<0.05或P<0.01)。LPS+血必净组与LPS+PDTC组比较,VEC中NO水平和iNOS蛋白表达水平差异无统计学意义(P>0.05),LPS+PDTC组VEC中NF-κB p65蛋白表达水平明显低于LPS+血必净组(P<0.05)。结论:血必净能抑制VEC中NO的产生和iNOS蛋白的表达,其机制可能是通过抑制NF-κB而抑制炎症反应。

关键词: 血必净;核转录因子-&kappa, B;诱导型一氧化氮合酶;一氧化氮;血管内皮细胞

Abstract:

Abstract:Objective To investigate the protective effects of Xuebijing(XBJ)on  the injury of vascular endothelial cells(VEC) induced by lipopolysaccharide (LPS), and to study the mechanisms of the production of nitric oxide (NO) and the expressions of inducible nitric oxide sytnhase (iNOS) and signal transduction under XBJ intervention condition. Methods   The cultured VEC were divided into control group, LPS(1 mg?L-1)group, LPS(1 mg/L) +XBJ(25 g/L)group,LPS(1 mg/L)+pyrrolidine dithiocarbamate(PDTC, 20 μmol/L)group; XBJ and PDTC were  administrated 1 h before incubation of with LPS. Western blotting method was used to detect the expressions of iNOS and NF-κB p65 protein. The level of  NO in the supernatant was measured by Griess reagent. Results   Comparaed with control group, the NO level and the expression levels of iNOS protein and NF-κB p65 protein in VEC in LPS group were significantly increased(P<0.01). Compared with LPS group, the NO level and the expression levels of iNOS protein and NF-κB p65 protein in VEC in LPS+XBJ group were significantly decreased (P<0.05 or P<0.01); the NO level and the expression levels of NF-κB p65 protein and iNOS protein in VEC in LPS+PDTC group were significantly decreased(P<0.05 or P<0.01). There were no significant differences of  the NO levels  and the expression levels of iNOS protein between LPS+XBJ group and LPS +PDTC group (P>0.05), but the expression level of NF-κB p65 protein in LPS+PDTC group was  lower than  that in LPS+XBJ  group(P<0.05). Conclusion  XBJ can inhibit the  production of NO and the expression of iNOS protein  in VEC; its  mechanism may be related to  inhibiting the activation of NF-κB to control inflammation.

Key words: Xuebijing, nuclear factor κB, inducible nitric oxide synthase, nitric oxide, vascular endothelial cells

中图分类号: 

  • R285.5