关键词: 遗传学, 多态性, 单核苷酸, 载脂蛋白D基因

Abstract: Abstract:Objective To investigate the genetic association between APOD gene and schizophrenia in the North Chinese Han population.Methods The PCR-based restriction fragment length polymorphism (RFLP) technique was conducted to examine the genotypes of rs2280250 locus of APOD gene in 291 family trios consisting of fathers,mothers,and affected offsprings with schizophrenia,and then the frequencies of genotype and allele of rs2280520 locus of APOD gene were statistically computed.Results ① The distribution of genotypic frequency of rs2280250 did not deviate from the Hardy-Weinberg equilibrium either in the patient group (χ²=0.178,df=1,P=0.673) or in the parent group (χ²=0.597,df=1,P=0.440); ②The transmission disquilibrium test (TDT) showed the allele transmitted from heterozygotic parents to affected offsprings did not bias from 50%(χ²=0.030,P=0.862); ③The haplotype-based haplotype relative risk test (HHRR) showed there was no significant difference of frequency distribution of allele of rs2280250 between patient group and parent group (χ²=0.055,P=0.815).Conclusion The polymorphism of rs2280250 at the APOD locus may not be associated with schizophrenia in North Chinese Han population.

Key words: genetics, polymorphism, single nucleotide, apolipoprotein D gene