硒蛋白P基因多态性与结直肠癌遗传易感性的关联性分析

doi:10.13481/j.1671-587x.20150518

吉林大学学报(医学版) ›› 2015, Vol. 41 ›› Issue (05): 980-985.doi: 10.13481/j.1671-587x.20150518

硒蛋白P基因多态性与结直肠癌遗传易感性的关联性分析

张龙, 朱燕, 王晓春, 张峻烽, 呼如芯, 李宇菲, 李春波

中南大学湘雅医学院医学检验系, 湖南长沙 410013

收稿日期:2014-12-25 出版日期:2015-09-28 发布日期:2015-09-29
通讯作者: 王晓春,教授,博士研究生导师(Tel:0731-82650279,E-mail:810768205@qq.com) E-mail:810768205@qq.com
作者简介:张龙(1989-),男,山东省枣庄市人,医学硕士,主要从事临床检验诊断学方面的研究。
基金资助:
湖南省科技厅科研项目资助课题(2014SK3102);湖南省卫生厅医药卫生科技项目资助课题(B2014-003);中南大学中央高校基本科研业务费专项基金资助资助课题(2014zzts326);中南大学2014年本科生自由探索项目资助课题(2282014bks195)

Analysis on correlation between genetic susceptibility of colorectal cancer and polymorphisms of selenoprotein P

ZHANG Long, ZHU Yan, WANG Xiaochun, ZHANG Junfeng, HU Ruxin, LI Yufei, LI Chunbo

Department of Medical Laboratory, Xiangya School of Medicine, Central South University, Changsha 410013, China

Received:2014-12-25 Online:2015-09-28 Published:2015-09-29

摘要/Abstract

摘要：

目的:探讨硒蛋白P(Sepp1)基因rs7579位点单核苷酸多态性(SNPs)与结直肠癌遗传易感性的关系,为结直肠癌的遗传学机制研究提供理论依据。方法:采用病例对照研究方法,采用四引物扩增阻碍突变体系聚合酶链反应(tetra-primer ARMS-PCR)技术检测130例结直肠癌患者及153名体检健康者Sepp1基因rs7579位点基因型和等位基因频数,并经测序验证。分析Sepp1基因rs7579位点基因型、等位基因与结直肠癌遗传易感性的关联性,并分析不同临床特征结直肠癌患者Sepp1基因rs7579位点基因型与结直肠癌遗传易感性的关联性。结果:结直肠癌组和健康对照组研究对象Sepp1基因 rs7579 位点多态性分布情况均符合Hardy-Weinberg平衡(P>0.05),结直肠癌组患者和健康对照者Sepp1基因rs7579位点GG、GA和AA基因型频数及G、A等位基因频数比较差异无统计学意义(P>0.05)。分层分析,年龄≥55岁组中,结直肠癌组和健康对照者组研究对象rs7579位点GG、GA和AA基因型频数比较差异有统计学意义(χ²=3.228,P=0.050),结直肠癌组患者AA基因型频率明显低于健康对照组(χ²=3.228,P=0.050,OR=0.566,95%CI:0.320-0.999)。结论:Sepp1基因rs7579位点多态性与结直肠癌的发病无关联,A等位基因可能是55岁以上中国人群降低结直肠癌发病的保护性因素之一。

关键词: 硒蛋白P, 结直肠肿瘤, 遗传易感性, 单核苷酸多态性

Abstract:

Objective To investigate the correlation between the polymorphisms of rs7579 site at selenoprotein P(Sepp1) gene and the genetic susceptibility of colorectal cancer,and to provide the theoretical basis for studying the genetic mechanism of colorectal cancer. Methods A case-control study was used in this research.The genotypic and allelic frequencies of Sepp1 gene were detected both in 130 patients with colorectal cancer and 153 healthy people by tetra-primer amplification refractory mutation system-polymerase chain reaction(tetra-primer ARMS-PCR)method,which was confirmed by gene sequencing analysis.The association between genotypic and allelic frequencies of rs7579 site at Sepp1 gene and genetic susceptibility of colorectal cancer was analyzed,then the association between the genotypes of rs7579 site at Sepp1 gene and genetic susceptibility of colorectal cancer in the patients with colorectal cancer with different clinical features was also analyzed. Results The distribution of polymorphism of rs7579 site at Sepp1 gene did not deviate from Hardy-Weinberg equilibrium in both case and control groups(P>0.05).There were no significant differences of the genotypic and allelic frequencies of rs7579 site at Sepp1 gene site between case group and control group(P>0.05).The stratification analysis results showed that there was significant difference of the genotypic frequency of rs7579 site between case group and control group in the patients more than 55 years old(χ²=3.228,P=0.050),and the genotypic frequency of AA of the patients in case group was significantly lower than that in control group(χ²=3.228,P=0.050,OR=0.566,95%CI:0.320-0.999). Conclusion The polymorphism of rs7579 site at Sepp1 gene is irrelevant to the pathogeny of colorectal cancer.Allele A may be one of the protective factors that reduce the morbidity of colorectal cancer among Chinese people older than 55 years old.

Key words: selenoprotein P, colorectal neoplasms, genetic susceptibility, single nucleotide polymorphism

中图分类号:

R735.3

张龙, 朱燕, 王晓春, 张峻烽, 呼如芯, 李宇菲, 李春波. 硒蛋白P基因多态性与结直肠癌遗传易感性的关联性分析[J]. 吉林大学学报(医学版), 2015, 41(05): 980-985.

ZHANG Long, ZHU Yan, WANG Xiaochun, ZHANG Junfeng, HU Ruxin, LI Yufei, LI Chunbo. Analysis on correlation between genetic susceptibility of colorectal cancer and polymorphisms of selenoprotein P[J]. Journal of Jilin University Medicine Edition, 2015, 41(05): 980-985.

参考文献

[1] Meplan C,Dragsted LO,Ravn-Haren G,et al.Association between polymorphisms in glutathione peroxidase and selenoprotein P genes,glutathione peroxidase activity,HRT use and breast cancer risk[J].PLoS One,2013,8(9):e73316.

[2] Geybels MS,Hutter CM,Kwon EM,et al.Variation in selenoenzyme genes and prostate cancer risk and survival[J].Prostate,2013,73(7):734-742.

[3] Geybels MS,van den Brandt PA,Schouten LJ,et al.Selenoprotein gene variants,toenail selenium levels,and risk for advanced prostate cancer[J].J Natl Cancer Inst,2014,106(3):u3.

[4] 张龙,王晓春.硒蛋白家族基因变异与胃肠道肿瘤遗传易感性的关联性研究进展[J].广东医学,2014,35(7):1128-1131.

[5] 张龙,王晓春.硒蛋白P基因多态性研究进展[J].生命科学研究,2014,18(5):441-444.

[6] Krol MB,Gromadzinska J,Wasowicz W.SeP,ApoER2 and megalin as necessary factors to maintain Se homeostasis in mammals[J].J Trace Elem Med Biol,2012,26(4):262-266.

[7] Saito Y,Sato N,Hirashima M,et al.Domain structure of bi-functional selenoprotein P[J].Biochem J,2004,381(Pt 3):841-846.

[8] Kurokawa S,Eriksson S,Rose KL,et al.Sepp1(UF) forms are N-terminal selenoprotein P truncations that have peroxidase activity when coupled with thioredoxin reductase-1[J].Free Radic Biol Med,2014,69:67-76.

[9] Hill KE,Zhou J,Mcmahan WJ,et al.Deletion of selenoprotein P alters distribution of selenium in the mouse[J].J Biol Chem,2003,278(16):13640-13646.

[10] Shetty SP,Shah R,Copeland PR.Regulation of selenocysteine incorporation into the selenium transport protein,selenoprotein P[J].J Biol Chem,2014,289(36):25317-25326.

[11] Meplan C,Crosley LK,Nicol F,et al.Genetic polymorphisms in the human selenoprotein P gene determine the response of selenoprotein markers to selenium supplementation in a gender-specific manner (the SELGEN study)[J].FASEB J,2007,21(12):3063-3074.

[12] Meplan C,Nicol F,Burtle BT,et al.Relative abundance of selenoprotein P isoforms in human plasma depends on genotype,se intake,and cancer status[J].Antioxid Redox Signal,2009,11(11):2631-2640.

[13] Sutherland A,Kim DH,Relton C,et al.Polymorphisms in the selenoprotein S and 15-kDa selenoprotein genes are associated with altered susceptibility to colorectal cancer[J].Genes Nutr,2010,5(3):215-223.

[14] 张龙,黄玉梅,李春波,等.硒蛋白S基因多态性与结直肠癌遗传易感性的相关性[J].临床检验杂志,2014,32(7):528-531.

[15] 杨四宝,刘雪岩,李冰,等.吉林汉族人群ERAP1基因3'UTR单核苷酸多态性与原发性高血压的关联性分析[J].吉林大学学报:医学版,2015,41(2):383-388.

硒蛋白P基因多态性与结直肠癌遗传易感性的关联性分析

Analysis on correlation between genetic susceptibility of colorectal cancer and polymorphisms of selenoprotein P

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