关键词: 可溶性血管内皮生长因子受体, 细胞增殖, 细胞凋亡, 裸鼠

Abstract: Objective To observe the effects of target solubility kinase insert domain receptor(sKDR) on proliferation and apoptosis of human bladder cancer T24 cells and the feasibility and effectiveness of sKDR gene as the target of bladder cancer gene therapy .Methods Human bladder cancer T24 cells were divided into sKDR transfected group,untransfected group and contol group.Eukaryotic secretory expression plasmid PCl-sKDR was constructed and T24 cells were transfected by Lipofectamine^TM2000 and further screened by ELISA . The expressions of sKDR gene in at all groups were determined by RT-PCR,the proliferation of T24 cells in these groups was measured by MTT method,the apoptotic rates were detected by TUNEL.T24 cells treated with sKDR were transplanted subcutanuously in nude mice and the tumorgenesis ability was observed.Results The expression of KDR mRNA in the KDR- expression T24 cells were 4.16 times that in vacant vector P^CI-neo cells.Compared with P^CI-neo contol,the proliferatory inhibitory rate of cells with sKDR- expression was increased by 55.1％(P<0.01).The tumorgenesis in transfected group was significantly restrained compared with untransfected group(P<0.01).Conclusion sKDR can inhibit the proliferateion and promote the apoptosis of bladder cancer T24 cells to a certain degree.

Key words: solubility kinase insert domain receptor, cell proliferation, apoptosis, nude mice