关键词: 白细胞介素18, 白细胞介素12, T淋巴细胞, 细胞毒性

Abstract: To compare the efficiency of IL-18 and IL-12 in inducing the tumor specific CTL in vitro,and test the cytotoxicities of tumor specific CTL induced in the same culture system with IL-18 or IL-12. MethodsThe NK cells,T cells and dendritic cells were separated from fresh PBMNCs by using the Stem Sep^TM immunomagnetic beads.Cell phenotypes of the purified cell populations were identified with FCM technique.The cell co-culture system was established as follows:the cell preparations in which definite cell subset had been deleted was co-cultivated with mitotic-inactivated tumor cells in the presence of rhIL-18 and/or rhIL-12.Cytotoxicities of the various effector cell preparations to a series of tumor cell lines were examined by the isotope releasing assay.Results In the  cell co-culture system in vitro,rhIL-18 or rhIL-12 alone and dose-dependently induced tumor specific cytotoxicities.The level of cytolytic activity of the tumor specific CTL induced by IL-12 was substantially inferior to that induced by IL-18 (P<0.01). ConclusionThese results suggest that IL-12 does not induce tumor-specific CTL as efficiently as IL-18 does in this co-culture system under these conditions，but the synergistic effect could be observed during administration of IL-18 and rIL-12.

Key words: interleukin-18, interleukin-12, T-lymphocytes, cytotoxic