关键词: 病毒非结构蛋白质类, 肝肿瘤

Abstract: Objective To investigate the correlation between the nonstructural protein 3（NS3）of chronic genotype 1b hepatitis C virus（HCV） and development of hepatocellular carcinoma (HCC). MethodsThe sera were collected from HCV-carriers and HCV-infected Chinese patients with and without HCC,HCV-RNA extracted from these serum samples were amplified by nested polymerase chain reaction (nested-PCR) to obtain the genotype 1b and NS3 region PCR products,GENETYX-Mac ver 10.1 software was used to analysis the nucleotide sequence,amino acid sequence and the secondary structure of NS3_1027-1206.Results The genotype 1b became predominant in all the patients,and the percentage had no statistically significant difference (P>0.05).The homology of nucleotide and amino acid sequence was 95%,there was no particular mutation in the NS3_1027-1206 region amino acid sequence.On the basis of secondary structure of NS3_1027-1206,the HCV isolates were classified into two types:type A and type B;type A was predominant in the carriers and non-HCC patients,type B was predominant in the HCC patients.The difference in the distribution pattern of those types between carriers and non-HCC patients was no statistically significant (P>0.05),but was statistically significant（P<0.01）between carriers,non-HCC patients and HCC patients.Conclusion There is difference in the secondary structure of NS3_1027-1206of carriers,non-HCC and HCC patients,and HCV-NS3 has correlation with the development of HCC.

Key words: viral nonstructural proteins, liver neoplasms