关键词: S_180<, sub>, Hep-A-22, Bcap-37, 肿瘤抑制作用

Abstract: Abstract:Objective To study the inhibitory effects of total flavonoids of scutellaria baicalensis georgi (TFSB) on S₁₈₀, Hep-A-22 and Bcap-37 tumor cell proliferation in vitro and on S₁₈₀, Hep-A-22 in mice bearing tumor in vivo.Methods In vitro, S₁₈₀, Hep-A-22 and Bcap-37 cells were divided into control group and TFSB groups (12.5，25.0，50.0，100.0 mg•L^-1).The inhibitory effects of TFSB on proliferation of S₁₈₀ and Hep-A-22 were measured by XTT colorimetric assay, and Bcap-37 cells were measured by MTT colorimetric assay.In vivo, the mice bearing tumor were divided into control group, CTX group (30 mg•kg^-1)，high, middle, low doses TFSB groups (200, 100，50 mg•kg^-1).After the mice bearing S₁₈₀ and Hep-A-22 tumor cells were treated with TFSB for 15 d, the tumor weights were measured, the inhibitory rates of S₁₈₀ and Hep-A-22 were calculated and survival of Hep-A-22 was measured after administration of TFSB for 10 d.Results TFSB inhibited the proliferation of S₁₈₀, Hep-A-22 and Bcap-37 cells, IC₅₀ values were 16.04, 17.74 and 9.05 mg•L^-1, respectively.The tumor weight of mice bearing S₁₈₀ and Hep-A-22 cells in TFSB groups(200, 100, 50mg•kg^-1) were lowered than that in control( P<0.01 or P<0.05). The tumor inhibitory rates were increased in dose-dependent manner.The survival of mice bearing Hep-A-22 in TFSB groups were higher than that in control（P<0.01 ).Conclusion TFSB may possess significantly inhibitory effect on proliferation of S₁₈₀, Hep-A-22 and Bcap-37 cells in vitro and the growth of tumor in mice bearing S₁₈₀ and Hep-A-22 in vivo.

Key words: S_180<, sub>, Hep-A-22, Bcap-37