关键词: 细胞死亡调节因子, 免疫组织化学, 逆转录聚合酶链反应

Abstract: Abstract:Objective To explore the expression of cell death regulatory gene GRIM-19 in human renal clear cell carcinoma tissues and normal renal tissues and significance,and clarity the effect of GRIM-19 in the process of renal clear cell carcinoma.Methods The expressions of GRIM-19 were studied by means of immunohistochemistry in 21 renal clear cell carcinoma tissues and 7 normal renal tissues. RT-PCR was used to detect the mRNA levels of GRIM-19 in 4 normal renal tissues and 8 renal clear cell carcinoma tissues. Results The results of immunohistochemistry indicated that the positive rates of GRIM-19 were 52.3% （11/21）and 100% （7/7） in renal clear cell carcinoma tissues and normal renal tissues,the difference was significant(P<0.05) . The positive rates of GRIM-19 were 100%（6/6），40%（4/10）and 20%（1/5）in the grade Ⅰ,Ⅱand Ⅲ of renal clear cell carcinoma,the differences between them were significant(P<0.01).RT-PCR results indicated that the positive rates of GRIM-19 were 62.5% （5/8）and 100% （4/4）in renal clear cell carcinoma tissues and normal renal tissues,the difference between them was significant（P<0.01）.Conclusion The expression of GRIM-19 obviously decrease in renal clear cell carcinoma tissue in the levels of gene and protein and the decreasing exists relevance with the grade of pathology. It indicates that GRIM-19 expression may be play an important role in the progress of renal clear cell carcinoma.

Key words: cell death regulatory gene, immunohistoche mistry, reverse transcriptase polymerase chain reaction