关键词: 生长抑素受体亚型, 生长抑素

Abstract: Abstract:Objective To detect the expressions of somatostatin receptor subtypes SSTR2 and SSTR5 mRNA in patients with hepatocellular carcinoma (HCC) and investigate the relationship between expression rates and carcinoma characters,in order to provide theoretical foundation for diagnosis and therapy of HCC. Methods 22　 patients with HCC were included,all the specimens with HCC were identified by surgery and the histopathological examination. The expressions of SSTR2 and SSTR5 mRNA in carcinoma tissues,adjacent tissues,and normal tissues were determined and identified with RT-PCR.The relationship between the expression rate and carcinoma characters was analyzed. Results The expression rate of SSTR2 mRNA in carcinoma tissues was 81.8%,the expression level was 118.27±17.25,they were obviously higher than those in normal tissues(36.4%，69.49±19.43) (P<0.05);but there was no difference between carcinona tissues and adjacent tissues(72.7%，111.18±23.74) (P> 0.05);there was obvious difference between normal tissues and adjacent tissues(P<0.05).There was no significant difference of the SSTR5 mRNA expression rates and expression levels between carcinoma tissues(18.2%， 51.67±30.32),adjacent tissues (18.2%，49.56±27.61) and normal tissues(9.1%，48.73±15.72)(P> 0.05). The expression rates of SSTR2 mRNA and SSTR5 mRNA in high differentiation HCC tissues(100% and 25%) and lymph node metastasis negtive HCC tissues(25.0% and SSTR5 100%)were higher than those in low differentiation HCC tissues(0 and 0) and lymph node metastasis positive HCC tissues(0 and 0)（P<0.05）；there was no obvious difference of 　the expression rates between TNM stageⅠ+ⅡHCC tissues(SSTR2 85.7%，SSTR5 21.4%) and TNM stage Ⅲ+Ⅳ(75.0%，12.5%)（P>0.05）. Conclusion The expression level of SSTR2 mRNA in HCC is high.The expression rate and expression level of SSTR2 have close relationship with the differentiantion,lymph node metastsis,and have no correlation with HCC classification.

Key words: somatostatin receptor subtype, somatostatin