重组人促卵泡激素对雄激素致不孕大鼠局灶性脑缺血脑皮质RAS相关基因表达的影响

doi:国家自然科学基金资助课题（30570687）

重组人促卵泡激素对雄激素致不孕大鼠局灶性脑缺血脑皮质RAS相关基因表达的影响

周家文^1,2，张勇¹，王皓^*，李洪岩¹，石国英¹，钟加滕¹，李扬¹，孙连坤¹，康劲松¹

(1.吉林大学基础医学院病理生理学教研室,吉林长春 130021;2.吉林大学中日联谊医院妇产科，吉林长春 130033 )

收稿日期:2008-10-13 修回日期:1900-01-01 出版日期:2009-03-28 发布日期:2009-08-14
通讯作者: 康劲松

Effect of recombinant human follitropin on expression of genes related to renin-angiotensin system in cortex of androgen-induced sterile rats with middle cerebral artery occlusion

ZHOU Jia-wen^1,2,ZHANG Yong¹,WANG Hao^*,LI Hong-yan¹,SHI Guo-ying¹,ZHONG Jia-teng¹,LI Yang¹,SUN Lian-kun¹,KANG Jin-song¹

(1. Department of Pathophysiology，School of Basic Medical Sciences,Jilin University,Changchun 130021,China； 2. Department of Obstetrics and Gynecology,China-Japan Union hospital，Jinlin University,Changchun 130033，China)

Received:2008-10-13 Revised:1900-01-01 Online:2009-03-28 Published:2009-08-14
Contact: KANG Jin-song

摘要/Abstract

摘要： 目的：探讨体内性激素的改变对局灶性脑缺血脑皮质肾素-血管紧张素（RAS）相关基因表达的影响。方法：建立雄激素致不孕大鼠（ASR）模型，给予果纳芬（rhFSH）后应用线栓法建立大鼠大脑中动脉阻塞(MCAO)模型。大鼠随机分为ASR+假手术（sham）组、ASR+rhFSH+sham组、ASR+MCAO组及ASR+rhFSH +MCAO组，每组12只。2，3，5 - 氯化三苯基四氮唑（TTC）法检测大脑的缺血面积；逆转录聚合酶链反应（RT-PCR）检测脑皮质血管紧张素原（AGT）、血管紧张素转换酶（ACE）、血管紧张素受体1（AT1）和血管紧张素受体2（AT2）的mRNA表达。结果：TTC染色显示，ASR+MCAO组及ASR+rhFSH+MCAO组大鼠大脑均可见苍白色的缺血区域。ASR+MCAO组及ASR+rhFSH+MCAO组脑梗死面积均明显大于ASR+sham组及ASR+rhFSH+sham组（P<0.05），但ASR+rhFSH+MCAO组脑组织梗死面积明显小于ASR+MCAO组(P<0.05)。与ASR+sham组比较，ASR+rhFSH+sham组的AGT、ACE、AT1及AT2 mRNA表达均无明显改变（P>0.05）；而ASR+MCAO组大脑皮质缺血侧ACE、AT1及AT2 mRNA的表达均明显增高(P<0.05)，但AGT未见明显改变。与ASR+rhFSH+sham组比较，ASR+rhFSH+MCAO组缺血侧大脑皮质AGT、ACE、AT1及AT2 mRNA的表达均无明显改变（P>0.05）。结论：雄激素的增高通过激活RAS系统参与了缺血性神经细胞的损伤，而rhFSH通过下调RAS系统减轻脑缺血损伤。

关键词: 大脑中动脉, 雄激素致不孕大鼠, 肾素-血管紧张素系统

Abstract: Abstract:Objective To explore the effect of sex hormone on genes related to rennin-angiotensin system(RAS) in cortex of androgen-induced sterile rats(ASR) with middle cerebral artery occlusion(MCAO). Methods The androgen-induced sterile rat model was first made,then administered with recombinant human follitropin （rhFSH）.The MCAO model was also established after rhFSH treatment.The rats were divided into four group:ASR+sham,ASR+ rhFSH+sham,ASR+MCAO,ASR+rhFSH+MCAO.There were 12 rats in each group.The brain ischemia area was measured by TTC staining. The AGT,ACE,AT1 and AT2 mRNA expressions in cortex were assayed by RT-PCR. Results TTC staining result showed that the pale rat brain ischemia areas were detected in ASR+MCAO and ASR+rhFSH+MCAO groups.The ischemia areas in ASR+MCAO group and ASR+rhFSH+MCAO group were larger than those in ASR+sham group and ASR+rhFSH+sham group （P<0.05）.The ischemia area in ASR+rhFSH+ MCAO group was smaller than that in ASR+MCAO group(P<0.05).There were no differences in AGT,ACE,AT1 and AT2 mRNA expressions between ASR+sham and ASR+rhFSH+sham groups（P>0.05）.The ACE,AT1 and AT2 mRNA expressions in ischemia side in ASR+MCAO group were obviously higher than those in ASR+sham group（P<0.05）,but the AGT mRNA did not change（P>0.05）.There were no differences in AGT,ACE,AT1 and AT2 mRNA expressions in ischemia side between ASR+rhFSH +MCAO group and ASR+rhFSH+sham group（P>0.05）.Conclusion The activation of RAS induced by androgen increasing is involved in brain ischemia injury.rhFSH can lessen the brain ischemia injury by down-regulating RAS.

Key words: middle cerebral artery, androgen-induced sterile rats, renin-angiotensin system

中图分类号:

R743.3

周家文，张勇，王皓，李洪岩，石国英，钟加滕，李扬，孙连坤，康劲松. 重组人促卵泡激素对雄激素致不孕大鼠局灶性脑缺血脑皮质RAS相关基因表达的影响[J]. J4, 2009, 35(2): 238-241.

ZHOU Jia-wen,ZHANG Yong,WANG Hao,LI Hong-yan,SHI Guo-ying,ZHONG Jia-teng,LI Yang,SUN Lian-kun,KANG Jin-song. Effect of recombinant human follitropin on expression of genes related to renin-angiotensin system in cortex of androgen-induced sterile rats with middle cerebral artery occlusion[J]. J4, 2009, 35(2): 238-241.

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