关键词: α亚基, 血管内皮生长因子A, 缺血肢体, 动脉粥样硬化闭塞症

Abstract: Abstract:Objective To investigate the expressions of hypoxia inducible factor-1α（HIF-1α） and vascular endothelial growth factor （VEGF） in vessels and muscle of ischemia lower limbs in patients with arteriosclerosis obliterans(ASO) in order to provide a theoretical basis for HIF-1α gene therapy in ischemic disease.Methods The ischemia lower limbs from 15 patients sufferring from ASO were used in the study with those from 3 normal individuals as control.The expressions of HIF-1α and VEGF in vessels and muscle were detected by immunohistochemistry (SP).The neovessels were marked with CD34,and microvessel density(MVD) was demonstrated by counting the number of positive cells of blood vessel endothelium marked CD34.Results The expressions of HIF-1α and VEGF in vessels and muscle of ischemic limbs were augmented obviously,accompanying with increase of MVD,especially in the posterior tibial artery(PTA).Density of HIF-1α,VEGF and MVD in the PTA were 932.5±545.2,354.5±75.8,and 38.4±8.4 separately.On the other hand,the expressions of HIF-1α and VEGF as well as MVD were less obvious in superficial femoral artery,anterior tibial artery,the vessels of ischemic muscular tissue of calf,the muscular tissue around necrotic tissue of feet compared with those in PTA.In ischemia vessels,the expressions of HIF-1α and VEGF and MVD increased obviously with the ischemia degree.On the contrary,the expressions of HIF-1α and VEGF and MVD in ischemia muscle were not related to ischemia degree.The expressions of HIF-1α and VEGF in ischemia muscle were less than those in ischemia vessels.Conclusion The　 expressions of HIF-1α and VEGF both in vessels and in muscle tissues of ischemia limbs in ASO patients are higher than those in normal tissues.The expressions of HIF-1α and VEGF in ischemia muscle are obviously lower than those in ischemia vessels.

Key words: alpha subunit, vascular endothelial growth factor A, ischemic extremity, arlerisclerosis obliterans