关键词: 调节性T细胞, 发病, 预后

Abstract: Abstract:Objective To study the expression of CD4⁺CD25^high Foxp3⁺ regulatory T cells (Tregs) in peripheral blood monouclear cell(PBMC) of patients with cancer,and explore the significance of Tregs in pathogenesis of cancer.Methods The proportions of CD4⁺CD25⁺/ CD4⁺,CD4⁺CD25^high / CD4⁺T cells in PBMC were examined with flow cytometry in 61 patients with cancer at different stages,and the expression levels of Foxp3⁺ in CD4⁺CD25⁺ and CD4⁺CD25^high T cells were analyzed,and compared with 32 normal control samples.Results Compared　 with normal controls,the proportions of CD4⁺CD25⁺/ CD4⁺,CD4⁺CD25^high / CD4⁺T cells in PBMC of patients with cancer at stageⅠand stage Ⅱ had no significant difference(P>0.05);but in those patients at stage Ⅲ and stage Ⅳ,they were higher than those in normal controls,the difference was significant(P<0.05).The proportions of CD4⁺CD25⁺/ CD4⁺,CD4⁺CD25^high / CD4⁺T cells were higher in PBMC of patients with cancer with higher histophathological grade.The expression levels of Foxp3⁺ in CD4⁺CD25⁺ and CD4⁺CD25^high T cells in patients with cancer were also higher than those in normal controls(P<0.01),the differences were significant(P<0.01). Conclusion The increase of Tregs in patients with cancer may be related to tumor progression and it shows immunological abnormality in patients with cancer.The regular detection of CD4⁺CD25⁺,CD4⁺CD25^high and CD4⁺CD25^high Foxp3⁺ in PBMC will contribute to understanding the patient’s condition and judging prognosis.

Key words: regulatory T cells, incidence, prognosis