神经生长因子对小鼠神经胶质细胞代谢及功能的影响

J4 ›› 2009, Vol. 35 ›› Issue (3): 487-490.

神经生长因子对小鼠神经胶质细胞代谢及功能的影响

王以薇¹, 张巨², 曹永安¹, 汪平¹

1.江苏职工医科大学生物化学教研室|江苏　南京 210029；２.吉林大学中日联谊医院手外科,吉林长春 130033

收稿日期:2008-10-13 出版日期:2009-05-28 发布日期:2009-08-14
通讯作者: 王以薇 E-mail:yiwei_w@126.com
作者简介:王以薇（1954-）|女|江苏省江阴市人|副教授|主要从事细胞生物学研究。
基金资助:
吉林省科技厅科技发展计划项目资助课题（20030544-1）

Effects of nerve growth factor on metabolism and function of neuroglial cells of mice

WANG Si-Wei¹, ZHANG Ju², CAO Yong-An¹, HONG Ping¹

1．Department of Biochemistry|Jiangsu Staff Medical University,Nanjing 210029, |China； 2．Department of Hand Surgery|China-Japan Union Hospital|Jilin University|Changchun 130033|China

Received:2008-10-13 Online:2009-05-28 Published:2009-08-14

摘要/Abstract

摘要：

目的：探讨神经生长因子(NGF)对正常小鼠及非肥胖性糖尿病(NOD)小鼠神经胶质细胞代谢及功能的影响。方法：实验设正常小鼠和NOD小鼠二组，每组10只，提取每只鼠神经胶质细胞后正常小鼠和NOD小鼠各分别分为5组，对照组（加RPMI-1640培养液）和4个实验组（每组10孔），实验组分别加入含5、10和20 μg·L^-1NGF及20 μg·L^-1　LPS +10 μg·L^-1NGF的培养液，分组培养。用黄嘌呤氧化酶、TBA法检测细胞培养液中SOD活性及MDA含量。结果：与正常小鼠对照组比较，NOD小鼠对照组SOD活性、MDA含量明显降低（P<0.01）；与NOD小鼠对照组比较，NOD小鼠各实验组神经胶质细胞SOD活性明显升高（P<0.05），MDA含量显著降低（P<0.05）。结论：NGF对促进NOD小鼠细胞代谢和改善细胞功能有一定作用。

关键词: 神经生长因子；神经胶质细胞；过氧化物歧化酶；丙二醛；非肥胖性糖尿病小鼠

Abstract:

Abstract:Objective To investigate the effects of nerve growth factor (NGF) on metabolism and function of neuroglial cells in the normal mice and the nonobese diabetic (NOD) mice.Methods The mice were divided into normol group and NOD group (n=10).The neuroglial cells abstracted from the normal mice and the NOD mice were randomly divided into control (with RPMI-1640) and 4 experimental groups (n=10);5,10 and 20 μg·L^-1NGF and 20 μg·L^-1LPS +10 μg·L^-1NGF were used in experimental groups.The activity of superoxide dismutase(SOD) and malonic dialdehyde(MDA) content in the culture solution were detected with xanthine oxidase and TBA methods.
Results Compared with control group of normal mice,the activity of SOD and MDA content in neuroglial cells of the NOD mice were significantly　decreased (P<0.01)；compared with that of respective control group,the activities of SOD were increased (P<0.05)and the MDA contents were decreased significantly (P<0.05) in the NOD mice of NGF groups.
Conclusion NGF has some effects on the improvement of cell metabolism and function in the NOD mice.

Key words: nerve growth factor；neuroglial cells； superoxide dismutase；malonic dialdehyde； nonobese diabetic mice

中图分类号:

王以薇, 张巨, 曹永安, 汪平. 神经生长因子对小鼠神经胶质细胞代谢及功能的影响[J]. J4, 2009, 35(3): 487-490.

WANG Si-Wei, ZHANG Ju, CAO Yong-An, HONG Beng. Effects of nerve growth factor on metabolism and function of neuroglial cells of mice[J]. J4, 2009, 35(3): 487-490.