人重组骨形成蛋白-7对人牙周膜成纤维细胞增殖及分化能力的影响

J4 ›› 2009, Vol. 35 ›› Issue (4): 677-680.

人重组骨形成蛋白-7对人牙周膜成纤维细胞增殖及分化能力的影响

蒋连权¹, 陈曦², 蔡绿树, 刘建林¹, 戴娟³

1.浙江大学附属第二医院口腔科|浙江杭州310006；2.西安交通大学医学院附属第一医院口腔科|陕西西安710061；3.第四军医大学口腔医学院正畸科|陕西西安 710032

收稿日期:2009-04-08 出版日期:2009-07-28 发布日期:2009-08-24
通讯作者: 戴娟 E-mail:daijuan@hotmail.com
作者简介:蒋连权（1974-）|男|浙江省慈溪市人|主治医师|主要从事牙周组织改建的生物学机制的研究。
基金资助:
陕西省科技厅科技计划项目资助课题（2003k102G103）；浙江省卫生厅医药卫生科学研究基金资助课题（2005B079）

Effect of recombinant human bone morphogenetic protein-7 on proliferation and differentiation |of human periodontal ligament cells

JIANG Lian-Quan¹, CHEN Xi², CAI Lu-Shu, LIU Jian-Lin¹, DAI Juan³

1.Department of Stomatology,Second Affiliated Hospital,Zhejiang University|Hangzhou 310006,China|2.Department of Stomatology|First Affiliated Hospital|Medical College,Xi’an Jiaotong University|Xi’an 710061,China；3.Department of Orthodontics,School of Stomatology,Fourth Military Medical University,Xi’an 710032,China

Received:2009-04-08 Online:2009-07-28 Published:2009-08-24

摘要/Abstract

摘要：

目的:研究人重组骨形成蛋白-7（ rhBMP-7）对体外培养的人牙周膜成纤维细胞(HPDLCs)增殖、分化能力的影响,为进一步探讨其促进牙周组织再生奠定细胞学基础。方法：选取因阻生或正畸需要拔除的牙齿，无菌条件下刮取根中1/3的牙周膜组织，采用组织块法培养牙周膜成纤维细胞，经免疫细胞化学鉴定后接种培养板，加入含浓度为50、100、200及400 μ mol·L^-1 rhBMP-7的培养液。分别于培养后1、3及5 d，采用MTT法测定HPDLCs的增殖能力，采用ELISA检测HPDLCs培养液中ALP的活性。结果:原代牙周膜细胞生长缓慢，加入rhBMP-7后细胞生长旺盛，细胞形态为纺锤形或成纤维样，胞浆透明，生长状态良好。随着时间的延长，rhBMP-7在100、200及400 μ mol·L^-1 各浓度时，HPDLCs细胞的增殖率呈逐渐增加的趋势(P<0.01)，但各浓度组间比较差异无显著性(P>0.05)；
与对照组比较，100、 200及400 μ mol·L^-1 rhBMP-7 各浓度组HPDLCs细胞裂解液和培养液中的ALP活性均增高(P<0.05或P<0.01)，其中200 μ mol·L^-1 rhBMP-7 组ALP活性最高。结论: BMP-7可能通过促进HPDLCs细胞的增殖和向骨细胞分化功能而参与牙周组织改建。

关键词: 骨形成蛋白-7；人牙周膜成纤维细胞；牙周组织改建

Abstract:

Abstract:Objective To study the effect of recombinant human bone morphogenetic protein-7 (rhBMP-7) on proliferation and differentiation of human periodontal ligament cells (HPDLCs) cultivated in vitro. Methods The HPDLCs were tested Vimentin positive and Ck (pan) negative.Different dosages (50,100,200 and 400 μmol·L^-1 )　 of rhBMP-7 were added in the cultured HPDLCs．1,3,5 d after administration of rhBMP-7,the proliferation and osteogenic differentiation of HPDLCs were examined by MTT assay and the activity of alkaline phosphatase(ALP) was detected with ELISA.
Results The administration of rhBMP-7 significantly elevated the proliferation rate and differentiation of HPDLCs with the dosage of l00,200 and 400 μmol·L^-1 respectively (P<0.01),no difference was found between different dosages groups(P>0.05)．Moreover,rhBMP-7 significantly induced the osteogenic differentiation ability with the increased expression level of ALP and 200 μmol·L^-1 was the most effective dosage in the differentiation ability.
Conclusion BMP-7 may participate in the remodeling of periodontal tissue by regulating the proliferation and osteogenic differentiation of HPDLCs.

Key words: bone morphogenetic protein-7;human periodontal ligament cells;remodeling of periodontal tissue

中图分类号:

蒋连权, 陈曦, 蔡绿树, 刘建林, 戴娟. 人重组骨形成蛋白-7对人牙周膜成纤维细胞增殖及分化能力的影响[J]. J4, 2009, 35(4): 677-680.

JIANG Lian-Quan, CHEN Xi, CAI Lu-Shu, LIU Jian-Lin, DAI Juan. Effect of recombinant human bone morphogenetic protein-7 on proliferation and differentiation |of human periodontal ligament cells[J]. J4, 2009, 35(4): 677-680.