哮喘鼠白细胞介素23水平变化及其作用机制

J4 ›› 2009, Vol. 35 ›› Issue (4): 681-684.

哮喘鼠白细胞介素23水平变化及其作用机制

张云峰¹, 赵凯姝¹, 张波¹, 孙萌², 鲁继荣¹

1. 吉林大学第一医院儿内一科|吉林长春 130021；2. 山东省青岛市儿童医院|山东青岛 266000

收稿日期:2009-04-15 出版日期:2009-07-28 发布日期:2009-08-24
通讯作者: 赵凯姝 E-mail:zhaoks@jlu.edu.com
作者简介:张云峰（1963-）|男|吉林省长春市人|副教授|副主任医师|医学博士|主要从事小儿呼吸及变态反应性疾病的研究。
基金资助:
吉林省科技厅科技发展计划项目资助课题（20080444-1）

hange of interleukin-23 level in mouse asthma model and its action mechanism

ZHANG Yun-Feng¹, DIAO Kai-Shu¹, ZHANG Bo¹, SUN Meng², LU Ji-Rong¹

1. Department of Pediatrics|First Hospital|Jilin University|Changchun 130021|China；2.Qingdao Children′s Hospital|Shandong Province,Qingdao 266000|China

Received:2009-04-15 Online:2009-07-28 Published:2009-08-24

摘要/Abstract

摘要：

目的：通过检测哮喘鼠血清白细胞介素23（IL-23）在激素治疗前后的水平变化及与IL-4、IFN-γ、IL-17的相关性，探讨其在哮喘发病中的作用。方法：将30只健康雌性BALB/C小鼠按完全随机设计原则分为3组，每组10只，即OVA致敏并激发的哮喘模型组（A 组）、鸡卵白蛋白(OVA)致敏、激发并予激素治疗组（B组）和正常对照组（C 组）。末次激发24 h后，各组小鼠摘眼球取血，ELISA法检测血清IgE、IL-4、IFN-γ、IL-17和IL-23的含量。结果：① 小鼠的行为学改变、血清IgE水平增高以及 IL-4 显著增高而 IFN-γ降低、IL-4与IFN-γ呈负相关等，均证明哮喘模型制备成功。② A组小鼠血清中IL-23 的水平［(155.56±12.50) ng·L^-1］ 高于C组［(51.49±6.34) ng·L^-1］，而B组小鼠血清IL-23 的水平［(88.00±6.43) ng·L^-1］低于A组（P＜0.01）。③ IL-23与IL-17含量成正相关关系(r= 0.763，P＜0.05)，与IL-4、IFN-γ均无相关性。结论：IL-23参与哮喘的发病，糖皮质激素对其有抑制作用。IL-23可能是通过诱导IL-17的产生间接促进哮喘发病，IL-23对于Th1 类细胞因子IFN-γ及 Th2 类细胞因子IL-4均无明显调节作用。

关键词: 哮喘；白细胞介素23；白细胞介素4；γ干扰素；白细胞介素17；糖皮质激素

Abstract:

Abstract:Objective To measure the change of serum interleukin-23 (IL-23) level before and after treatment with glucocortcoid and analyze the correlations between levels of IL-23 and IL-4， IFN-γ，IL-17, and explore the role of IL-23 in bronchial asthma.
Methods Thirty femal BALB/c mice were randomly divided into three group (n=10): group A (asthma model), group B (corticosteroid treatment) and group C (normal control). All mice were killed 24 h after final OVA challenge.The blood samples were obtained for measurements of serum IgE，IL-4，IFN-γ,IL-17and IL-23 levels by enzyme-linked immunoabsorbent assay (ELISA).
Results The mouse asthma model appeared ethological changes specific to asthma.The level of OVA specific IgE in serum increased.The level of serum IL-4 increased and the level of IFN-γ decreased.Correlation analysis showed negative relationship between IL-4 and IFN-γ（r=－0.859，P＜0.01），there existed Th1／Th2 imbalance.The level of serum IL-23 in group A was higher than that in group C (P＜0.01). In mice treated with inhaled budesonide (group B)，the level of serum IL-23 was lower than that in group A (P＜0.01).The concentration of IL-23 had no correlation with both IL-4 and IFN-γ.There was a positive correlation between the levels of serum IL-23 and IL-17 (r=0.763，P＜0.01).
Conclusion IL-23 in serum might be involved in the pathogenesis of bronchial asthma，and could be inhibited by inhaled glucocorticoid. IL-23 has no relationship with IFN-γ and IL-4. It suggested that IL-23 might contribute to the development of asthma indirectly by inducing IL-17 generation.

Key words: bronchial asthma；interleukin-4；interferon-γ；interleukin-17；interleukin-23； , glucocorticoid

中图分类号:

R-332

张云峰, 赵凯姝, 张波, 孙萌, 鲁继荣. 哮喘鼠白细胞介素23水平变化及其作用机制[J]. J4, 2009, 35(4): 681-684.

ZHANG Yun-Feng, DIAO Kai-Shu, ZHANG Bo, SUN Meng, LU Ji-Rong. hange of interleukin-23 level in mouse asthma model and its action mechanism[J]. J4, 2009, 35(4): 681-684.