MMP-9基因多态性与IgA肾病临床表现的关联性分析

J4 ›› 2009, Vol. 35 ›› Issue (4): 690-693.

MMP-9基因多态性与IgA肾病临床表现的关联性分析

孙珉丹¹, 罗萍², 耿兴花¹

1.吉林大学第一医院泌尿系统疾病诊治中心|吉林长春 130021；2.吉林大学第二医院肾
内科|吉林长春 130041

收稿日期:2009-04-08 出版日期:2009-07-28 发布日期:2009-08-24
通讯作者: 罗萍 E-mail:Luopingjen@163.com
作者简介:孙珉丹（1967-）|女|吉林省九台市人|副主任医师|副教授|医学博士|主要从事肾小球疾病发病机理的研究。
基金资助:
吉林省科技厅科研基金资助课题（200505218）

Analysis of association between clinical manifestation and polymorphism of matrixmetalloproteinase-9 C-1562T gene in patients with IgA nephropathy

SUN Min-Dan¹, LUO Ping², GENG Xing-Hua¹

1.Department of Nephrology,First Hospital,Jilin University,Changchun 130021,China;2.Department of Nephrology,Second Hospital,Jilin University,Changchun 130041,China

Received:2009-04-08 Online:2009-07-28 Published:2009-08-24

摘要/Abstract

摘要：

目的：研究中国长春地区汉族IgA肾病患者的MMP-9 C-1562T基因多态性与临床表现的关系。方法：应用聚合酶链反应-限制性片段长度多态性分析(PCR-RELP)技术检测85例IgA肾病患者的MMP-9C -1562T基因多态性，应用SPSS １３.０分析基因多态性与前驱感染、肉眼血尿、肾区疼痛、高血压、肾功能改变、是否表现为肾病综合征及尿蛋白定量等临床表现的关联性。结果：IgA肾病患者MMP-9 C-1562T基因型分布频数为：CC基因型62例
（72.94%），CT基因型23例（27.06%），TT基因型缺如,符合Hardy-Weinberg平衡定律;ＣＣ和ＣＴ基因型与24 h尿蛋白定量有关联性(U=502.0，P=0.0454)，与前驱感染、肉眼血尿、肾区疼痛、高血压、肾功能改变、是否表现为肾病综合征及镜下血尿程度等临床表现无关（P>0.05）。结论：临床上IgA肾病患者的MMP-9 C-1562T基因型可能影响尿蛋白程度，但不影响其他临床表现，亦不决定其预后。

关键词: 基质金属蛋白酶-9;C-1562T基因；遗传多态性；IgA肾病；临床表现

Abstract:

Objective To study the relationship between matrixmetalloproteinase-9(MMP-9) C-1562T gene polymorphism and the clinical manifestations in patients with IgA nephropathy.Methods The polymorphism of MMP-9 C-1562T gene in 85 IgA nephropathy patients were detected with polymerase chain reation-restriction fragment length polymorphism(PCR-RELP),SPSS13.0 was used to analyze the correlations between the gene polymorphism and pre-infection,gross hematuria,renal area pain,hypertension,renal fuction change,confirmed to clinical features of nephritic syndrome or not and the degree of microscopic hematuria.Results The genetypic frequency distributions of MMP-9 C-1562T gene in IgA nephropathy patients were CC 65(72.94%),CT 23(27.06%),TT 0(0%),they were not deviated from Hardy-Weinbery equilibrium. The genotypes of IgA may be associated with urinary protein(U=502.0,P=0.0454),unassociated with pre-infection,gross hematuria,renal area pain,hypertension,renal fuction change,confirmed to clinical features of nephritic syndrome or not and the degree of microscopic hematuria（P>0.05）.Conclusion On clinical,MMP-9 C-1562T genotype of IgA nephropathy patients may impact on the degree of urinary protein,but not other clinical manifestations,and prognosis of IgA nephropathy.

Key words: matrixmetalloproteinase-9;C-1562T gene;genetic polymorphism;IgA nephropathy;clinical manifestation

中图分类号:

R345.2

孙珉丹, 罗萍, 耿兴花. MMP-9基因多态性与IgA肾病临床表现的关联性分析[J]. J4, 2009, 35(4): 690-693.

SUN Min-Dan, LUO Ping, GENG Xing-Hua. Analysis of association between clinical manifestation and polymorphism of matrixmetalloproteinase-9 C-1562T gene in patients with IgA nephropathy[J]. J4, 2009, 35(4): 690-693.