槲寄生碱对低分化骨肉瘤U2OS的抑制作用

J4 ›› 2009, Vol. 35 ›› Issue (5): 841-844.

槲寄生碱对低分化骨肉瘤U2OS的抑制作用

葛岩|王医术|高婷|张丽红|廖翔宇|郝艳艳|李玉林

吉林大学基础医学院　病理生物学教育部重点实验室|吉林长春 130021

收稿日期:2009-05-26 出版日期:2009-09-28 发布日期:2009-09-28
通讯作者: 李玉林；王医术 E-mail:ylli@jlu.edu.cn;wangys@jlu.edu.cn
作者简介:葛岩（1982-）|女|吉林省白城市人|在读医学博士|主要从事病理生物学方面的研究。
基金资助:
吉林省科技厅科技发展计划项目资助课题（200505107）；长春市科技局攻关项目资助课题（2006266）；吉林省中医药管理局资助课题（08SYS-068）

Inhibitory effect of mistletoe alkali on osteosarcoma cells

GE Yan, WANG Yi-Shu, GAO Ting, ZHANG Li-Hong, LIAO Xiang-Yu, HAO Yan-Yan, LI Yu-Lin

Key Laboratory of Pathobiology, Ministry of Education, Jilin University, Changchun　130021, China

Received:2009-05-26 Online:2009-09-28 Published:2009-09-28

摘要/Abstract

摘要：

目的：通过体外实验研究槲寄生碱对人低分化骨肉瘤细胞（U2OS）生长、侵袭的影响。方法：以5-氟尿嘧啶(5-FU)作用组为阳性对照组，以不加药组为阴性对照组，槲寄生组为实验组作用U2OS细胞，采用CCK-8试剂盒检测药物对体外培养的U2OS细胞生长抑制率。绘制在不同药物浓度作用下的细胞生长曲线。观察不同药物浓度作用下细胞的形态学变化。应用Boyden小室检测槲寄生碱对U2OS细胞侵袭能力的影响。结果：槲寄生碱作用U2OS细胞的IC50值为7 mg·L^-1；生长曲线显示，加药组细胞生长速度较未加药组明显缓慢，细胞由多角型逐渐变为圆形，排列疏松，附壁性减弱。Boyden小室实验显示，槲寄生碱作用后，迁移至聚碳酸脂膜下的细胞数(47.0±2.4)明显少于未加药组(122.0±12.1)（P<0.05）。结论：槲寄生碱在体外具有抑制人低分化骨肉瘤细胞U2OS细胞生长、侵袭的作用，有望成为治疗骨肉瘤的辅助药物。

关键词: 槲寄生碱；人低分化骨肉瘤；抑制；生长；肿瘤侵润

Abstract:

Abstract:Objective To study the inhibitory effect of mistletoe alkali on the growth and invasiveness of human osteosarcoma cells (U2OS) in vitro. Methods The effects of mistletoe alkali on the growth of U2OS cells were detected by CCK-8 kit. The U2OS cells treated by 5-FU were used as positive control group and the U2OS cells without any treatment as negative control group, the U2OS cells treated by mistletoe alkali were used as experimental groups. Meanwhile the growth of U2OS cells after treated by mistletoe alkali was assessed by cell growth curve. And the morphological changes of U2OS cells under different concentrations of medicine were observed. Furthermore, Their invasion ability was tested with Boyden chamber. Results Mistletoe alkali inhibited the growth of U2OS cells and the value of IC50 was 7 mg·L^-1. After treatment, the growth of U2OS cells slowed, and the cells became round, adherence decreased. Furthermore, the invasion ability of cells in the experiment group was obviously decreased. Conclusion Mistletoe alkali obviously inhibits the growth and the invasion ability of U2OS cells in vitro.

Key words: mistletoe alkali；osteosarcoma；inhibit；growth；neoplasm invasiveness

中图分类号:

R738

葛岩, 王医术, 高婷, 张丽红, 廖翔宇, 郝艳艳, 李玉林. 槲寄生碱对低分化骨肉瘤U2OS的抑制作用[J]. J4, 2009, 35(5): 841-844.

GE Yan, WANG Yi-Shu, GAO Ting, ZHANG Li-Hong, LIAO Xiang-Yu, HAO Yan-Yan, LI Yu-Lin. Inhibitory effect of mistletoe alkali on osteosarcoma cells[J]. J4, 2009, 35(5): 841-844.