人宫颈癌组织中CK17和P63蛋白的表达及意义

J4 ›› 2009, Vol. 35 ›› Issue (5): 914-917.

人宫颈癌组织中CK17和P63蛋白的表达及意义

张松灵¹, 张立红², 钟艳平³, 李玉林²

1. |吉林大学第一医院妇产科|吉林长春 130021；2.吉林大学基础医学院病理生物学教育部重点实验室|吉林长春 130021；3.吉林大学第一医院病理科|吉林长春 130021

收稿日期:2009-04-03 出版日期:2009-09-28 发布日期:2009-09-28
通讯作者: 李玉林 E-mail:ylli@jlu.edu.cn
作者简介:张松灵（1975-）|女|吉林省长春市人|医学博士|主治医师|主要从事妇科肿瘤诊治方面的研究。
基金资助:
教育部科学技术研究重点项目资助课题（01058）；吉林省科技厅科技发展计划项目资助课题（白求恩基金200705155）

Expressions of CK17 and P63 in human cervical cancer tissues and significances

ZHANG Song-Ling¹, ZHANG Li-Hong², ZHONG Yan-Ping³, LI Yu-Lin²

1.Department of Obstetrics and Gynecology,First Hospital,Jilin University,Changchun 130021,China;2.Key Laboratory of Pathobiology,Ministry of Education,Jilin University,Changchun 130021,China;3.Department of Pathology,First Hospital,Jilin University,Changchun 130021,China

Received:2009-04-03 Online:2009-09-28 Published:2009-09-28

摘要/Abstract

摘要：

目的：研究宫颈储备细胞标记物CK17及P63蛋白在人宫颈癌组织中的表达情况，判定其成为宫颈癌干细胞标记物的可能性。方法：采用免疫组织化学染色法检测55例人宫颈鳞状细胞癌组织及20例正常人宫颈组织中CK17及P63蛋白的表达情况。结果：① CK17蛋白表达于正常宫颈组织鳞柱交接的储备细胞胞浆；在宫颈癌组织中，CK17蛋白表达于部分宫颈癌细胞胞浆，表达部位主要位于瘤栓中、肿瘤浸润的前沿及边缘部位；在恶性度较高的宫颈癌中，CK17蛋白表达率明显高于恶性度较低的宫颈癌组（P<0.05）；在化疗后的标本组织中，CK17蛋白的表达率也较化疗前明显升高（P<0.05）。②P63蛋白表达于正常宫颈组织鳞柱交接的储备细胞的胞核中；在宫颈癌组织中，几乎所有的宫颈癌细胞均表达P63蛋白。结论：CK17蛋白在伴有转移、浸润、低分化及化疗后的病例中表达的阳性率明显升高，且趋于表达在瘤栓中、肿瘤浸润的前沿及边缘部位，可能是宫颈癌干细胞的标记物；P63蛋白几乎表达于所有宫颈癌细胞中，表达无规律性，可能不是宫颈癌干细胞的标记物。

关键词: 子宫颈；肿瘤, 鳞状细胞；CK17；P63；肿瘤干细胞；生物学标记

Abstract:

Abstract:Objective To investigate the expressions of CK17 and P63 in cervical cancer tissues,and explore the possibility of CK17 and P63 as markers of cervical cancer stem cells.Methods The expressions of CK17 and P63 in cervical squamous cancer tissues (n=55)　and normal cervix tissues (n=20) were detected with immunohistochemical method.Results ①CK17 expressed in reserve cell cytoplasm in normal cervical specimens.In the cervical cancer specimens,the expression of CK17 was increased and it seemed that CK17 was stained in the margin of the cancer nest and the tumor embolus.In specimens with high grade malignant cancer or after the chemotherapy,the expressions of CK17 were increased（P<0.05）.②P63 was stained in nuclei of reserve cells and almost all cancer cells.Conclusion CK17 is stained in the margin of the cancer nest and the tumor embolus.In specimens with high grade malignant cancer or after the chemotherapy,the expression of CK17 is increased.CK17 may be the marker of cervical cancer stem cells，but P63 not.

Key words: cervix uter；neoplasms,squamous cell；CK17；P63；neoplastic stem cells

中图分类号:

R711

张松灵, 张立红, 钟艳平, 李玉林. 人宫颈癌组织中CK17和P63蛋白的表达及意义[J]. J4, 2009, 35(5): 914-917.

ZHANG Song-Ling, ZHANG Li-Hong, ZHONG Yan-Ping, LI Yu-Lin. Expressions of CK17 and P63 in human cervical cancer tissues and significances[J]. J4, 2009, 35(5): 914-917.