抑癌基因PTEN单核苷酸多态性与喉癌的关系

J4 ›› 2009, Vol. 35 ›› Issue (5): 918-922.

抑癌基因PTEN单核苷酸多态性与喉癌的关系

宋朝霞¹|祝威²|李鹏¹|刘冰¹

1. 吉林大学基础医学院医学遗传学教研室|吉林长春 130021；2. 吉林大学第一医院耳鼻咽喉-头颈外科|吉林长春130021

收稿日期:2009-03-31 出版日期:2009-09-28 发布日期:2009-09-28
通讯作者: 刘冰 E-mail:liubingww@yahoo.com.cn
作者简介:宋朝霞(1971-)|吉林省长春市人|副教授|理学博士|主要从事肿瘤遗传学研究。
基金资助:
吉林省科技厅科研基金资助课题（200505187）

Association between anti-oncogene PTEN single nucleotide polymorphism and laryngocarcinoma

SONG Zhao-Xia¹, ZHU Wei², LI Peng¹, LIU Bing¹

1.Department of Medical Genetics,School of Basic Medical Sciences,Jilin University,Changchun 130021,China；2. Department of Otorhinolaryngology,First Hospital,Jilin University|Changchun 130021,China

Received:2009-03-31 Online:2009-09-28 Published:2009-09-28

摘要/Abstract

摘要：

目的：探讨抑癌基因PTEN单核苷酸多态性与中国北方汉族人群喉癌的关系。方法：采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法，检测149例喉癌患者（病例组）和104名健康者（对照组）的PTEN基因rs1903858和rs701848位点的基因型，应用SPSS 10.0分析PTEN的基因多态性与喉癌的关系。结果：PTEN基因rs1903858和rs701848位点的基因型频数分布在病例组和对照组均符合Hardy-Weinberg平衡定律(P>0.05)；病例组rs1903858和rs701848两个SNPs等位基因及基因型的频数分布与对照组比较差异均无显著性（P>0.05）。结论：PTEN基因的rs2735343和rs701848基因多态性可能与中国北方汉族人群喉癌的发生无关。

关键词: PTEN；多态性,单核苷酸；喉肿瘤

Abstract:

Abstract:Objective To investigate the genetic association between the PTEN single polymorphisms and laryngocarcinoma in Chinese Han population.Methods The method of PCR-based restriction fragment length polymorphism (PCR-RFLP) analysis was used to detect the genotypes of rs1903858 and rs701848 of PTEN gene in 253 subjects,including 149 sporic laryngocarcinoma patients(case group) and 104 controls(control group).The association between anti-oncogene PTEN single nucleotide polymorphism and laryngocarcinoma was analyzed with SPSS10.0. Results The genotypic frequencies of the 2 SNPs of anti-oncogene PTEN did not deviate from Hardy-Weinberg equilibrium in both case and control groups.The allelic and genotypic frequencies of the 2 SNPs had no remarkable differences between case and control groups (P>0.05).Conclusion Neither rs1903858 nor rs701848 of the PTEN gene has no association with laryngocarcinoma in Chinese Han population.

Key words: phosphatase and tensin homologue deleted on chromosome ten;polymorphisms, single nucleotide； laryngeal neoplasms

中图分类号:

R739.65

宋朝霞, 祝威, 李鹏, 刘冰. 抑癌基因PTEN单核苷酸多态性与喉癌的关系[J]. J4, 2009, 35(5): 918-922.

SONG Zhao-Xia, ZHU Wei, LI Peng, LIU Bing. Association between anti-oncogene PTEN single nucleotide polymorphism and laryngocarcinoma[J]. J4, 2009, 35(5): 918-922.