肿瘤细胞对CD4+CD25+Treg细胞数量和功能的影响

J4 ›› 2009, Vol. 35 ›› Issue (6): 1002-1006.

肿瘤细胞对CD4+CD25+Treg细胞数量和功能的影响

李欣^1,2, 杨巍¹, 付海英¹, 张佳伦¹, 李一¹

(1.吉林大学基础医学院免疫学教研室|吉林长春 130021；2.长春中医药大学微生物与免疫学教研室|吉林长春 130117)

收稿日期:2009-08-06 出版日期:2009-11-28 发布日期:2009-11-28
通讯作者: 李一 E-mail:Tel:0431-85619541，E-mail:yilili19@yahoo.com.cn
作者简介:李欣（1979-）|女|吉林省临江市人|医学博士|主要从事肿瘤免疫耐受的研究。
基金资助:
国家自然科学基金资助课题（30872307）

Influence of tumor cells in number and function of CD4+CD25+Treg cells

LI Xin^1,2, YANG Wei¹, FU Hai-Yang¹, ZHANG Jia-Lun¹, LI Yi¹

（1.Department of Immunology,School of Basic Medical Sciences,Jilin University,Changchun 130021,China；2.Department of Immunology and Microbiology,Changchun University of Traditional Chinese Medicine,Changchun 130117,China）

Received:2009-08-06 Online:2009-11-28 Published:2009-11-28

摘要/Abstract

摘要：

目的：探讨肿瘤细胞与免疫细胞相互作用对CD4+CD25+Treg细胞数量和功能的影响。方法：建立Lewis肺癌细胞与小鼠脾淋巴细胞共培养体系。Lewis肺癌细胞与不同浓度小鼠脾淋巴细胞共培养，分为4组：实验组Ⅰ（5×105个Lewis肺癌细胞与1×106个淋巴细胞共培养）、对照组Ⅰ（1×106个淋巴细胞单独培养）、实验组Ⅱ（5×105个Lewis肺癌细胞
与2×106个淋巴细胞共培养）、对照组Ⅱ（2×106个淋巴细胞单独培养）；Lewis肺癌细胞与
淋巴细胞共培养不同时间，采用3个时间点：24、48和72 h；Lewis肺癌细胞培养上清与淋巴细
胞共培养，选择培养上清浓度为20%和50%。采用流式细胞术检测了Lewis肺癌细胞与脾淋巴
细胞共培养系统中CD4+CD25+Treg细胞数量变化，通过RT-PCR方法检测了共培养对Foxp
3 mRNA表达的影响。结果：与对照组比较，实验组Ⅰ 中CD4+CD25+Treg细胞数量和Foxp3 mRN
A表达明显增强（P<0.05），实验组Ⅱ中CD4+CD25+Treg细胞数量和Foxp3 mRNA表达无明
显变化（P>0.05）；Lewis肺癌细胞与淋巴细胞培养24及48 h可见CD4+CD25+Treg细胞数量及Foxp3
mRNA表达明显升高（P<0.05），而72 h后变化不明显；20%和50% Lewis肺癌细胞培养上清
均可明显提高CD4+CD25+Treg数量及Foxp3 mRNA表达（P<0.05）。结论：肿瘤细胞及其培养
上清可诱导CD4+CD25+Treg细胞数量增加、功能增强，由肿瘤细胞所引起的CD4+CD25+Treg细
胞产生及功能增强可能是肿瘤逃避免疫监视机制之一。

关键词: CD4+CD25+Treg细胞；Foxp3；肿瘤免疫

Abstract:

To explore the influence of tumor cells in the number and function of CD4+CD25+Treg cells.
Methods Lewis lung cancer cells and mouse spleen lymphocytes co-culture system was established.Lewis lung cancer cells with different concentrations of lymphocytes co-cultured were divided into 4 groups:experimental group Ⅰ(5×105 Lewis lung cancer cells and 1×106 lymphocytes c
o-culture),control group Ⅰ(1×106 lymphocytes culture),experimental group Ⅱ(5×105 Lewis lung cancer cells and 2×106 lymphocytes co-culture),control group Ⅱ(2×106 lymphocytes culture);Lewis lung cancer cells were co-cultivated with lymphocytes for different time, 24,48, and 72 h three time points were selected.Lewis lung cancer cells culture supernatant was co-cultivated with lymphocytes,the concentrations of culture supernatant were 20% and 50%.The number changes of CD4+CD25+Treg cells in the co-culture system of Lewis lung cancer cells and splenic lymphocytes were detected by flow cytometry;the expression of Foxp3 mRNA after co-culture was detected by RT-PCR method.
Results Compared with control group,the number of CD4+CD25+Treg cells and the expression of Fo
xp3 mRNA were significantly increased in experimental group Ⅰ (P<0.05),and ther
e was no significant difference in experimental group Ⅱ (P>0.05);24　and 48 h 　
after co-culture of Lewis lung cancer cells and lymphocytes,the number of CD4
+CD25+Treg cells and the expression of Foxp3 mRNA were significantly increased (P<0.05),
and there was no significant changes at 72 h;20% and 50% Lewis lung cancer cells supernatant could significantly increase the number of CD4+CD25+Treg cells and Foxp3 mRNA expression (P<0.05).Conclusion Tumor cells and their supernatants could induce the increase of the number of CD4+CD25+Treg cells and their function,this might be one of mechanisms of tumor-induced immune tolerance.

Key words: CD4+CD25+Treg cells;Foxp3;tumor immunity

中图分类号:

R393

李欣, 杨巍, 付海英, 张佳伦, 李一. 肿瘤细胞对CD4+CD25+Treg细胞数量和功能的影响[J]. J4, 2009, 35(6): 1002-1006.

LI Xin, YANG Wei, FU Hai-Yang, ZHANG Jia-Lun, LI Yi. Influence of tumor cells in number and function of CD4+CD25+Treg cells[J]. J4, 2009, 35(6): 1002-1006.