金刚烷胺修饰物对禽流感病毒感染小鼠免疫功能的影响

J4 ›› 2009, Vol. 35 ›› Issue (6): 1011-1014.

金刚烷胺修饰物对禽流感病毒感染小鼠免疫功能的影响

徐坤¹, 李静¹, 李金华¹, 侯雷¹, 杨松涛², 王宏芳¹, 浦昀¹, 吴新宇¹, 李娟¹, 夏咸柱²

（1.吉林大学公共卫生学院卫生检验教研室|吉林长春 130021； 2. 解放军军事医学科学院军事兽医研究所|吉林长春 130062）

收稿日期:2009-06-01 出版日期:2009-11-28 发布日期:2009-11-28
通讯作者: 李娟 E-mail:Tel：0431-85619419,E-mail：li_juan@jlu.edu.cn
作者简介:徐坤（1979-）|男|黑龙江省海伦市人|在读医学博士|主要从事化合物抗病毒研究。
基金资助:
吉林省科技厅科研基金资助课题（200705389）；国家“十一五”重大新药创制科技重大专项基金资助课题(2009ZX09103-105)

Effect of modified amantadine on immune function of mice infected with influenza A(H5N1) virus

XU Kun¹, LI Jing¹, LI Jin-Hua¹, HOU Lei¹, YANG Song-tao², WANG Hong-Fang¹, PU Yun¹, WU Xin-Yu¹, LI Juan¹, JIA Xian-Zhu²

(1.Department of Health Laboratory,School of Public Health,Jilin University,Changchun 130021,China|2. Institute of Military Veterinary,Academy of Military Medical Sciences,Changchun 130062,China)

Received:2009-06-01 Online:2009-11-28 Published:2009-11-28

摘要/Abstract

摘要：

目的：观察金刚烷胺修饰物（NAM）对禽流感病毒感染小鼠免疫功能的影响，为抗禽流感病毒新药NAM的开发提供实验依据。方法：采用禽流感病毒（H5N1）感染小鼠模型，实验分正常对照组（NC）、模型组（PC）、金刚烷胺阳性药物组（AMA）及NAM 25、50、100和200 mg?kg-1剂量组。测定小鼠胸腺指数、脾指数、刺激指数(SI)、NK细胞杀伤活性和干扰素（IFN）活性。结果： NAM 100 mg?kg-1剂量组平均脾指数高于PC组(P<0.05)；NAM各剂量组平均胸腺指数高于PC组(P<0.05)。NAM 25、100和200 mg?kg-1剂量组的刺激指数高于PC组(P<0.05)；NAM 100和200 mg?kg-1剂量组刺激指数高于AMA组(P<0.05)。NAM 100和200 mg?kg-1剂量组NK细胞杀伤活性高于PC组(P<0.05)；NAM 25、100和200 mg?kg-1剂量组NK细胞杀伤活性高于AMA组(P<0.05)。NAM 50、100和200 mg?kg-1剂量组IFN活性高
于PC组(P<0.01)； NAM 50、100和200 mg?kg-1剂量组IFN活性高于AMA组(P<0.05)。结论：NAM可刺激禽流感病毒感染小鼠脾细胞和胸腺细胞增殖，对T淋巴细胞转化功能、NK细胞杀伤活性及IFN免疫活性均有不同程度的促进作用。

关键词: 禽流感病毒；金刚烷胺；免疫功能

Abstract:

Abstract:Objective To explore the effect of modified amantadine(NAM) on immune function of the mice infected with influenza A(H5N1) virus and to provide experimental basis for development of NAM.Methods Mouse models infected with influenza A (H5N1) virus were established.And the mice were divided into 7 groups:normal control group（NC）,positive control group (PC),amantadine group (AMA) and 4 NAM groups (which contained 25,50,100 and 200 mg?kg-1 NAM, respectively).The spleen and thymus index,T lymphocyte transformation,NK cytotoxicity activity and interferon (IFN) activity of mice were detected.Results The mean spleen index in 100 mg?kg-1 NAM group was higher than that in PC group (P<0.05),and the mean thymus index in NAM group was higher than that in PC group (P<0.05).The spleen indexes in 25,100 and 200 mg?kg-1 NAM groups were increased compared with PC group(P<0.05),and were and increased in 100 and 200 mg?kg-1 NAM groups compared with AMA group (P<0.05).The NK cytotoxicity activities in 100 and 200 mg?kg-1 NAM groups were higher than that in PC group(P<0.05),while they were also higher in 25,100 and 200 mg?kg-1 NAM groups than that in AMA group (P<0.05).The IFN activities in 50,100 and 200 mg?kg-1NAM groups were increased compared with PC group(P<0.05),and the IFN activities in 50,100 and 200 mg?kg-1 NAM groups were also increased compared with AMA group (P<0.05).
Conclusion NAM can stimulate the proliferation of spleen and thymus cells of mice infected with influenza A(H5N1) virus,and enhance T lymphocyte transformation,NK cytotoxicity and IFN activity.

Key words: avian influenza virus;amantadine;immune function

中图分类号:

R978.7

徐坤, 李静, 李金华, 侯雷, 杨松涛, 王宏芳, 浦昀, 吴新宇, 李娟, 夏咸柱. 金刚烷胺修饰物对禽流感病毒感染小鼠免疫功能的影响[J]. J4, 2009, 35(6): 1011-1014.

XU Kun, LI Jing, LI Jin-Hua, HOU Lei, YANG Song-tao, WANG Hong-Fang, PU Yun, WU Xin-Yu, LI Juan, JIA Xian-Zhu. Effect of modified amantadine on immune function of mice infected with influenza A(H5N1) virus[J]. J4, 2009, 35(6): 1011-1014.