聚肌胞对小鼠前列腺癌组织内血管生成的影响

J4 ›› 2009, Vol. 35 ›› Issue (6): 1029-1031.

聚肌胞对小鼠前列腺癌组织内血管生成的影响

田原僮¹, 钟毅², 曾靖¹, 赵雪俭³, 赵丽娟³

1.赣南医学院药理学教研室|江西赣州 341000；2.江西省赣县人民医院泌尿外科|江西赣
县　341100；3.吉林大学基础医学院病理生理学教研室| 吉林长春 130021）

收稿日期:2009-07-13 出版日期:2009-11-28 发布日期:2009-11-28
通讯作者: 赵丽娟 E-mail:titan2003@sina.com.cn
作者简介:田原僮（1979-）女|吉林省通化市人|讲师|医学硕士|主要从事肿瘤药理学研究。
基金资助:
科技部国际科技合作重点项目资助课题（2004DFB02000）

Effect of polyi:c on angiogenesis in mouse prostate carcinoma tissue

TIAN Yuan-tong¹, ZHONG Yi², ZENG Jing¹, ZHAO Xue-Jian³, ZHAO Li-Juan³

(1. Department of Pharmacology,Gan Nan Medical School,Ganzhou 341000,China|2.Department of Urinary Surgery,People’s Hospital of Ganxian,Ganxian 341100,China;3.Department of Pathophysiology, School of Basic Medical Sciences,Jilin University,Changchun 130021,China)

Received:2009-07-13 Online:2009-11-28 Published:2009-11-28

摘要/Abstract

摘要：

目的：探讨聚肌胞对小鼠前列腺癌组织内血管生成的影响，阐明其可能机制。方法：将16只C57BL6/J纯系荷瘤小鼠按瘤重随机分为对照组和聚肌胞组，分别于瘤内注射生理盐水和聚肌胞(1次?3　d-1)，用药7次后切除瘤灶，计算瘤重抑制率和瘤指数。肿瘤组织HE染色，镜下观察前列腺癌组织形态学变化及血管分布情况。肿瘤组织血管内皮生长因子（VE
GF）、内皮型一氧化氮合酶（eNOS）蛋白免疫组织化学染色，观察其蛋白表达情况。结果：聚肌胞组小鼠前列腺癌的瘤重抑制率为67.85%，瘤指数为（5.42±0.17）%；对照组瘤指数为（14.45±1.06）%，两组比较差异有显著性( P<0.01)。聚肌胞组和对照组前列腺癌组织中VEGF阳性染色肿瘤细胞计数占同类肿瘤细胞的比例分别为(9.64±2.90)% 和(37.91±7.62)%，eNOS阳性染色肿瘤细胞计数占同类肿瘤细胞的比例分别为（22.00±8.07）%和（54
.43±10.39）%，两组比较差异均有显著性(P<0.01)。结论：聚肌胞通过降低小鼠前列腺癌组织中VEGF和eNOS蛋白的表达，抑制小鼠前列腺癌组织内血管的生成，减少肿瘤组织供
氧和养分，对小鼠前列腺癌产生间接抑制作用。

关键词: 聚肌胞；前列腺肿瘤；血管内皮生长因子；内皮型一氧化氮合酶

Abstract:

Abstract:Objective To investigate the effects of polyi:c on angiogenesis in mouse prostate carcinoma tissue and their possible mechanisms.Methods Mouse prostate carcinoma models were randomly divided into two groups according to tumor weight:control group and polyi:c group.After treatment for 7 times,the mice were sacrificed and the tumor tissues were cut for weighing,calculating the tumor inhibitory rate and tumor index.Hematoxylin-eosin staining and immunohistochemical staining were performed to observate the morphological changes of prostate carcinoma tissues,distribution of vasa and expressions of vascular endothelial growth factor (VEGF) and endothelial nitric-oxide synthase (eNOS).
Results In polyi:c group,the mean tumor inhibitory rate was 67.85% and the tumor index was（5.42±0.17）%;in control group,the tumor index was（14.45±1.06）%; there was significant differences between polyi:c group and control group( P<0.01). The expressions of VEGF in polyi:c group and control group were (9.64±2.90)% and (37.91±7.62)%, respectively,and there was significant difference between two groups( P<0.01). The expressions of eNOS in polyi:c group and control group were (4.43±10.39)% and (22.00±8.07)%，respectively,and there was significant difference between two groups ( P<0.01).Conclusion Through decreasing the expressions of VEGF
and eNOS,polyi:c plays an important role in inhibitting the angiogenesis and indirect inhibiting mouse prostate carcinoma.

Key words: polvriboinsine-polyribocyaidylic acid;prostaic neoplasms;vascular endothelial growth factor;endothelial nitric-oxide synthase　　

中图分类号:

R73-36

田原僮, 钟毅, 曾靖, 赵雪俭, 赵丽娟. 聚肌胞对小鼠前列腺癌组织内血管生成的影响[J]. J4, 2009, 35(6): 1029-1031.

TIAN Yuan-tong, ZHONG Yi, ZENG Jing, ZHAO Xue-Jian, ZHAO Li-Juan. Effect of polyi:c on angiogenesis in mouse prostate carcinoma tissue[J]. J4, 2009, 35(6): 1029-1031.