Bcl-2及Bax在异丙肾上腺素致心肌坏死模型大鼠心肌中的表达及意义

J4 ›› 2009, Vol. 35 ›› Issue (6): 1044-1047.

Bcl-2及Bax在异丙肾上腺素致心肌坏死模型大鼠心肌中的表达及意义

李相军¹|杨莉^1|2|任立群¹|石艳¹|孙波¹|苗春生¹

1.吉林大学药学院实验药理与毒理学教研室|吉林长春 130021；2 .内蒙古通辽市医院功能检查科|内蒙古通辽 028000

收稿日期:2008-12-23 出版日期:2009-11-28 发布日期:2009-11-28
通讯作者: 任立群 E-mail:renlq@jlu.edu.cn
作者简介:李相军(1974-）|男|吉林省吉林市人|讲师|医学博士|主要主要从事心血管疾病研究。
基金资助:
教育部博士点基金资助课题（200801830050）

Expressions of Bcl-2 and Bax in myocardium of rats with isoproterenol-induced myocardial necrosis and significances

LI Xiang-Jun¹, YANG Li^1|2, RIN Li-Qun¹, SHI Yan¹, SUN Bo¹, MIAO Chun-Sheng¹

1.Department of Experimental Pharmocology and Toxicology,School of Pharmacy,Jilin University,Changchun 130021,China；2.Department of Function Examination,Hospital of Tongliao City|Tongliao 028000,China

Received:2008-12-23 Online:2009-11-28 Published:2009-11-28

摘要/Abstract

摘要：

目的：探讨Bcl-2及Bax在异丙肾上腺素（Isp）致心肌坏死模型大鼠心肌中的表达及意义。方法：40只大鼠随机分为4组：正常对照组及注射Isp后12 h、1周和3周组。除正常对照组外，其他各组大鼠皮下注射Isp（15 mg?kg-1体重），并于给药后12 h、1周和3周时取对应组大鼠心脏，用免疫组化法及RT-PCR法检测各组大鼠心肌组织中Bcl-2及Bax的表达。结果：免疫组化结果显示，注射Isp后 12 h及1周组大鼠心肌组织中Bax表达明显高于正常对照组（P<0.05）；注射Isp后12 h、1周及3周组大鼠心肌组织Bcl-2表达量均明显低于正常对照组（P<0.05）；注射Isp后12 h、1周、3周后，各组大鼠心肌组织Bcl-2/Bax比值均低于正常对照组（P<0.05）。RT-PCR结果显示，注射Isp后各组大鼠心肌组织Bax mRNA表达量均明显高于正常对照组（P<0.05），且在注射Isp后1周时，Bax mRNA表达量达到高峰；注射Isp后，各组大鼠心肌组织Bcl-2 mRNA表达量均明显低于正常对照组大鼠（P<0.05）；与正常对照组大鼠比较，在注射Isp后 12h及1周，各组大鼠心肌组织Bcl-2/Bax比值明显增高（P<0.05）。结论：心肌细胞的凋亡是心肌纤维化的病理基础，而Bcl-2家族基因及蛋白表达变化在此过程中起主要调控作用。

关键词: 心肌坏死；细胞调亡；基因, Bcl-2；Bax

Abstract:

Objective To detect the expressions of Bcl-2 and Bax gene and protein in myocardium of rats with isoproterenol (Isp)-induced myocardial necrosis and significances.Methods 40 Wistar rats were divided into control group,12 h,1 week and 3 weeks after Isp injection groups.Except control group,all the rats in the other groups were treated with isoproterenol hydrochloride.12 h,1 week and 3 weeks after Isp injection,immunohistochemistry and RT-PCR were applied to detect the gene and protein expressions of Bcl-2 and Bax.Results The results of immunohistochemistry showed that 12 h and 1 week after Isp injection,the expressions of Bax were increased significantly compared with control group (P<0.05);Inversely,the expressions of Bcl-2 were decreased significantly 12 h,1 week and 3 weeks after Isp injection compared with normal control group (P<0.05).The ratios of Bcl-2/Bax were significantly decreased 12 h,1 week and 3 weeks after Isp injectioncompared with control group (P<0.05). The expressions of Bax mRNA were increased significantly
12 h,1 week and 3 weeks after Isp injection compared with control group (P<0.05);The expressions of Bcl-2 were decreased significantly 12 h and 1 week after Isp injection compared with control group (P<0.05).Conclusion Apoptosis of myocardium is the basic pathological change of myocardial fibrosis,and the expressions of gene and protein of Bcl-2 family play an important role in this process.

Key words: myocardial necrosis;apoptosis;gene,bcl-2;Bax

中图分类号:

R542.22

李相军, 杨莉, 任立群, 石艳, 孙波, 苗春生. Bcl-2及Bax在异丙肾上腺素致心肌坏死模型大鼠心肌中的表达及意义[J]. J4, 2009, 35(6): 1044-1047.

LI Xiang-Jun, YANG Li, RIN Li-Qun, SHI Yan, SUN Bo, MIAO Chun-Sheng. Expressions of Bcl-2 and Bax in myocardium of rats with isoproterenol-induced myocardial necrosis and significances[J]. J4, 2009, 35(6): 1044-1047.

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