关键词:  RNA结合基序蛋白； 转录介导融合基因；肿瘤细胞, 培养的

Abstract:

Objective
 To determine the existence of RBM6-RBM5 chimera and study its relationship with oncogenesis. Methods Ten cell lines including GLC-20,MDA.MB.231, Jurkat,TF-1,MCF-7,Hela,BT-474,A431,K562 and Raji ； several cDNAs from tumor tissues including lung,ovary,skeletal,skin,spleen,uterus,heart,bone,brain,lymph node,pancreas,prostate,testis  and their normal controls and 5 breast cancer tissues were collected for the study.Nested PCR was used to identify the existence and expression level of RBM6-RBM5 chimera.QPCR was used to determine the RBM6,RBM5 gene expression level in chimeric positive and negative tumor tissues.Results RBM6-RBM5 chimera was identified in MDA，MB，231 cell line,which lacked exons 20,21 of RBM6 and exon 1 of RBM5.The chimeric expression was only observed on 10 tumor cell lines and tissues,among some specific tumor types,the chimeric expression was associated with elevated levels of RBM6 and RBM5 mRNA.Conclusion  RBM6 mRNA experiences altered co-transcriptional gene regulation in certain cancers.RBM6-RBM5 transcription-induced chimerism might be a process that is linked to the tumor-associated increased transcriptional activity of the RBM6 and RBM5 genes,and this chimera might be a new marker for tumor prediction. 
〖WTHZ〗Key words：RNA binding motif protein;transcription-induced chimera;tumor cells,cultured

Key words: RNA binding motif protein;transcription-induced chimera;tumor cells,cultured