关键词: 哮喘；气道重构；疾病模型, 动物；变应原  

Abstract:

Abstract:Objective
To build an airway remodeling model of bronchial asthma in mouse  with  house dust mite extract (DHM)，and to investigate the dose-effect and time-effect relationship.Methods Ninety  SPF  BALB/c  mice  were randomly divided into control group,low dose DHM group and high dose DHM group.Each group was randomly divided into three subgroups:three-week,six-week and nine-week groups.The mice was challenged with intranasal drip after sensitized with subcutaneous and peritoneal injection of DHM with differet doses.All mice were underwent pulmonary lavage in 24 h after the last challenge.the total inflammation cell count,eosinophil count in BALF,the IL-4 level and total serum IgE level were detected,and the internal perimeter (Pi),the wall thickness(d) and wall area(WA) of the bronchi were measured by computer image analytical system.Results In both high dose DHM and low dose DHM groups,the total inflammation cell counts and eosinophil counts,the levels of IL-4 and serum total IgE levels were significantly increased (P<0.05);the d/Pi and WA/Pi were statistically increased (P<0.05) compared with control group.The airway inflammation was increased significantly since the third week,and there was incremental change of morphological parameters in  high dose DHM group during 9 weeks.Compared with low dose DHM group,in high dose DHM group,the total inflammation cell and eosinophil counts were increased in 3 weeks( P<0.05),the levels of IL-4 and IgE were  increased in 3,6 and 9 weeks (P<0.05), while the d/Pi and WA/Pi had no significant change.
Conclusion DHM can induce airway remodeling model of bronchial asthma in mouse successfully,and the airway remodeling changes obviously with the prolongation of exposured time,and there is significant dose- and time-effect in the study.

Key words: asthma;airway wall remodeling;disease models,animal;allergens