反义微小RNA-21对人膀胱癌细胞增殖和凋亡的影响

J4 ›› 2010, Vol. 36 ›› Issue (3): 450-452.

反义微小RNA-21对人膀胱癌细胞增殖和凋亡的影响

张明|魏巍|刘禄成|李志|温都苏

吉林大学第二医院泌尿外科|吉林长春130041

收稿日期:2009-11-18 出版日期:2010-05-28 发布日期:2010-05-28
通讯作者: 张明（Tel: 0431-88796972,E-mail:zhangm@163.com) E-mail:zhangm@163.com
作者简介:张明（1963-）|男|吉林省九台市人|教授|医学博士|主要从事泌尿系肿瘤治疗的研究。〖ZK)〗
基金资助:
国家自然科学基金资助课题（30972981）

Effect of antisense miRNA-21 on proliferation and apoptosis of bladder cancer cells

ZHANG Ming, WEI Wei, LIU Lu-Cheng, LI Zhi, WEN Dou-Su

Departement of Urinary Surgery,Second Hospital,Jilin University,Changchun 130041| China

Received:2009-11-18 Online:2010-05-28 Published:2010-05-28

摘要/Abstract

摘要：

目的：探讨微小RNA-21（miRNA-21）反义寡核苷酸（ASOND）对人膀胱癌细胞株T24增殖和凋亡的影响，为膀胱癌的治疗提供理论依据。方法：将脂质体介导的反义寡核苷酸（AS-miRNA-21）转染T24细胞，设转染无义序列组、对照组和AS-miRNA-21转染组，采用蛋白印迹(Western blotting)检测转染细胞miRNA-21表达水平，噻唑蓝（MTT）比色法和流式细胞仪（FCM）分别检测转染细胞的增殖和凋亡情况，转染无义序列组、对照组分别与AS-miRNA-21转染组比较。结果：经过转染AS-miRNA-21的T24细胞miRNA-21表达水平下降，细胞增殖能力受抑。AS-miRNA-21组T24细胞增殖率为53.6%，转染无义序列组T24细胞增殖率为92.5%，对照组为100%;相对应的凋亡率分别为13.8%、1.9%和2.7%。上述3组间T24细胞的增殖率、凋亡率比较差异均有显著性（P<0.01）。结论：反义miRNA-21对人膀胱癌细胞T24具有抑制增殖和促进凋亡的作用，抑制miRNA-21表达有望成为膀胱癌基因治疗的有效方法。

关键词: 膀胱肿瘤；微小RNA-21；反义寡核苷酸类

Abstract:

Abstract:Objective To investigate the effect of miRNA-21 antisense oligonucleotide（ASOND） on the proliferation and apoptosis of bladder cancer cell line T24,and provide theoretical basis for treatment of bladder cancer. Methods AS-miRNA-21 mediated by liposome was transfected into T24 cells. As-miRNA-21 group,transfection nonsense series group and control group were set up.The protein expression level of miRNA-21 of the transfected cells was detected by Western blotting,and the proliferation and apoptosis of the transfected cells were measured by MTT and FCM.Results The miRNA-21 expression level of the tumor cells transfected with AS-miRNA-21 was decreased,the cell proliferation was inhibited.The proliferation
rates of T24 cells in AS-miRNA-21 group,transfection nonsense series group and control group were 53.6%,92.5%,and 100%,respectively; the relative apoptotic rates were 13.8%,1.9% and 2.7%.There were significant differences of the proliferation rate and apoptotic rates between the three groups（P<0.01）.Conclusion Antisense miRNA-21 has the action of proliferation inhibition and apoptosis enhancement on human bladder cancer cell T24. Inhibiting the expression of miRNA-21 may become a prospective gene therapy for bladder cancer.

Key words: bladder neoplasms;miRNA-21;antisense oligonucleotides

中图分类号:

R735.14

张明, 魏巍, 刘禄成, 李志, 温都苏. 反义微小RNA-21对人膀胱癌细胞增殖和凋亡的影响[J]. J4, 2010, 36(3): 450-452.

ZHANG Ming, WEI Wei, LIU Lu-Cheng, LI Zhi, WEN Dou-Su. Effect of antisense miRNA-21 on proliferation and apoptosis of bladder cancer cells[J]. J4, 2010, 36(3): 450-452.