缺氧预处理对脑缺血大鼠脑电图的影响

J4 ›› 2010, Vol. 36 ›› Issue (4): 669-672.

缺氧预处理对脑缺血大鼠脑电图的影响

金海龙¹|徐雪²|李永旺³|王保国⁴

1.首都医科大学附属北京天坛医院麻醉科|北京 100050；2.河北医科大学二院麻醉科|河北石家庄 050051；3.第三军医大学附属新桥医院麻醉科|重庆 400037；4.北京三博脑科医院|北京 100093

收稿日期:2010-03-17 出版日期:2010-07-28 发布日期:2010-07-28
通讯作者: 王保国（Tel：010-62856788，E-mail：wbgttyy@sina.com） E-mail:wbgttyy@sina.com
作者简介:金海龙(1974-)|男|吉林省延吉市人|主治医师|医学博士|主要从事神经外科麻醉与脑保护研究。
基金资助:
重庆市自然科学基金资助课题（CSTC,2007BB5027）

Effect of hypoxic preconditioning on electroencephalogram in brain ischemic rats

JIN Hai-Long¹, XU Xue², LI Yong-Wang³, WANG Bao-Guo⁴

1.Department of Anesthesiology, Beijing Tiantan Hospital, Capital Medical University, Beijing100050, China|2. Department of Anesthesiology, Second Hospital, Hebei Medical University, Shijiazhuang 050051, China|3. Department of Anesthesiology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China|4. Beijing Sanbo Brain Institute, Beijing 100093,China

Received:2010-03-17 Online:2010-07-28 Published:2010-07-28

摘要/Abstract

摘要：

目的：观察缺氧预处理后脑缺血大鼠的脑电图(EEG)变化，探讨脑电活动改变在脑缺血耐受中的作用。方法：60只雄性Wistar大鼠被随机分为3组：①窒息心停跳组 (ACA组，n=20），大鼠遭受窒息性心跳停止，1 min后复苏；②缺氧预处理+窒息心停跳组 (HP+ACA组，n=20），在窒息性心停跳前24 h预先给予大鼠1 min的缺氧预处理4次，每次间隔5 min；③缺氧预处理组 (HP组，n=20），只给予4次缺氧预处理。观察各组大鼠窒息后心跳停止时间（TCA）、开始复苏后自主循环恢复时间（TROSC）、死亡率和复苏后神经功能评分 (NDS) ，记录预处理及缺血期间EEG变化。结果：ACA组和HP+ACA组大鼠死亡率分别为30%和5%（P<0.01）；与ACA组比较，HP+ACA组TCA明显延长而TROSC明显缩短(P<0.01)，复苏后24、48和72 h的NDS明显改善 (P<0.01)，窒息后EEG等电位时间、复苏后EEG出现时间和连续EEG出现时间均明显缩短 (P<0.05)。结论：缺氧预处理可通过调整大鼠脑电活动诱发脑缺血耐受作用。

关键词: 缺氧预处理；脑缺血；脑电图

Abstract:

Abstract:Objective To observe the electroencephalogram (EEG) changes of asphyxial cardiac arrest (ACA) after hypoxic preconditioning (HP) in rats and investigate the activity of EEG in brain ischemic tolerance. Methods Sixty male Wistar rats were randomly assigned to three groups: ①ACA group (n=20),the rats were subjected to 1 min of ACA and then resuscitated; ② HP+ACA group (n=20),the rats were pretreated with 4 times of 1 min HP 24 h before ACA;③HP group (n=20),the rats were treated with 4 times of 1 min HP. The mortality, EEG, time from initiation of asphyxia to CA (TCA) and time from resuscitation to restoration of spontaneous circulation (TROSC) were recorded; neuro-functional outcome was assessed by neurodeficit score (NDS). Results The mortality was 5% in HP+ACA group and 30% in ACA group (P<0.01); compared with ACA group, the rats in HP+ACA group showed much shorter TCA and significant longer TROSC（P<0.01）. NDS in HP+ACA group was greatly better than in ACA group (P<0.01); the time to EEG silence and restoration of EEG were significantly shorter in HP+ACA group (P<0.05). Conclusion Hypoxic preconditioning can induce brain ischemic tolerance through EEG activity modulation.

Key words: hypoxic preconditioning, brain ischemia, electroencephalogram

中图分类号:

R364.4

金海龙, 徐雪, 李永旺, 王保国. 缺氧预处理对脑缺血大鼠脑电图的影响[J]. J4, 2010, 36(4): 669-672.

JIN Hai-Long, XU Xue, LI Yong-Wang, WANG Bao-Guo. Effect of hypoxic preconditioning on electroencephalogram in brain ischemic rats[J]. J4, 2010, 36(4): 669-672.