紧密连接蛋白CLDN6在卵巢癌、肝癌以及脑膜瘤组织中的表达及意义

J4 ›› 2010, Vol. 36 ›› Issue (4): 698-702.

紧密连接蛋白CLDN6在卵巢癌、肝癌以及脑膜瘤组织中的表达及意义

刘亚芳¹|李久霞^1,2|张广超^1,3|吴琼¹|许晓明¹|任玥¹|于立娜¹|全成实¹|李玉林¹

1.吉林大学基础医学院病理生物学教育部重点实验室|吉林长春 130021；2. 白城医学高等专科学校|吉林白城 137000；3. 吉化总医院伽玛刀治疗研究中心| 吉林吉林132022

收稿日期:2009-10-22 出版日期:2010-07-28 发布日期:2010-07-28
通讯作者: 全成实(Tel:0431-85619481,E-mail:quanchengshi@yahoo.com) E-mail:quanchengshi@yahoo.com
作者简介:刘亚芳（1980-）|女|吉林省农安县人|在读医学博士|主要从事肿瘤病理生物学研究。
基金资助:
国家自然科学基金资助课题(30670807)；高等学科博士学科专项科研基金资助课题（20060183071）；吉林省科技厅科技发展计划项目资助课题（200505141）

Expressions of tight junctions protein CLDN6 in ovarian cancer,liver cancer and meningioma tissues and significances


LIU Ya-Fang¹, LI Jiu-Xia^1,2, ZHANG Guang-Chao^1,3, WU Qiong¹, XU Xiao-Ming¹, REN Yue¹, YU Li-Na,¹ QUAN Cheng-Shi¹, LI Yu-Lin¹

1. Key Laboratory of Pathobiology,Ministry of Education,School of Basic Medical Sciences,Jilin University,Changchun 130021,China；2. Baicheng Medical College|Baicheng 137000,China;3.Research |Center of Gamma Knife Treatment|Jihua Hospital,Jilin 132022|China

Received:2009-10-22 Online:2010-07-28 Published:2010-07-28

摘要/Abstract

摘要：

目的：探讨紧密连接蛋白CLDN6在卵巢癌、肝癌以及脑膜瘤组织中的表达及意义，阐明CLDN6在肿瘤发生、发展中的作用。方法：用免疫组织化学SP法检测36例卵巢癌、30例肝癌以及29例侵袭性脑膜瘤组织中紧密连接蛋CLDN6的表达，同时分别以26例卵巢良性肿瘤，19例癌旁正常肝组织以及29例非侵袭性脑膜瘤组织作为对照，分析CLDN6在上述肿瘤中的表达与临床指标之间的关系。结果：卵巢癌组织中CLDN6表达阳性率为69.44%，卵巢良性瘤中CLDN6表达阳性率为34.62%，两者比较差异具有显著性（P<0.05）。肝癌组织中CLDN6表达阳性率为73.33%，癌旁正常肝组织中CLDN6表达阳性率为26.31%，两者比较差异具有显著性（P<0.05）；侵袭性脑膜瘤组织中CLDN6表达阳性率为10.34%，非袭性脑膜瘤组织 CLDN6表达阳性率为65.51%，两者比较差具有显著性（P<0.05）。结论：CLDN6在卵巢癌和肝癌组织中表达上调，在侵袭性脑膜瘤组织中表达下调，推测CLDN6表达改变可能会破坏紧密连接组成成分的比例，进而在肿瘤的发生发展中起一定作用。

关键词: 肿瘤；紧密连接；CLDN6；免疫组织化学

Abstract:

Objective
To explore the CLDN6 expressions in ovarian cancer,liver cancer and meningioma tissues and clinical significances,and clarify its effects in the occurrence and development of cancer.Methods The expressions of CLDN6 in 36 ovarian cancer tissues,30 liver cancer tissues and 29 invasion meningioma tissues were detected by SP immunohistochemical staining,at the same time,26 benign neoplasm of ovarian tissues,19 normal hepatic tissues and 29 non-invasion meningioma tissues were used as control.Results The positive expression rates of CLDN6 in ovarian cancer tissues and benign neoplasm of ovarian tissues were 69.44% and 34.62%,there was significant difference between them（P<0.05）；the expression positive rates of CLDN6 in liver cancer tissues and normal hepatic tissues were 73.33% and 26.31%,there was significant difference between then(P<0.05);the positive expression rates of CLDN6 in invasion meningioma tissues and non-invasion meningioma tissues were 10.34% and 65.51%，there was also significant difference（P<0.05）.Conclusion The expression of CLDN6 is up-regulated in ovarian cancer and liver cancer tissues,and down-regulated in invasion meningioma tissues. It may have positive or negative regulatory effect on different cancers by destroying compose of tight junctions.

Key words: neoplasms；tight junctions；CLDN6；immunohistochemisty

中图分类号:

R361.2

刘亚芳, 李久霞, 张广超, 吴琼, 许晓明, 任玥, 于立娜, 全成实, 李玉林. 紧密连接蛋白CLDN6在卵巢癌、肝癌以及脑膜瘤组织中的表达及意义[J]. J4, 2010, 36(4): 698-702.

LIU Ya-Fang, LI Jiu-Xia, ZHANG Guang-Chao, WU Qiong, XU Xiao-Ming, REN Yue, YU Li-Na, QUAN Cheng-Shi, LI Yu-Lin. Expressions of tight junctions protein CLDN6 in ovarian cancer,liver cancer and meningioma tissues and significances
[J]. J4, 2010, 36(4): 698-702.