转录因子FOXD1在乳腺癌组织中的表达及意义

J4 ›› 2010, Vol. 36 ›› Issue (4): 703-707.

转录因子FOXD1在乳腺癌组织中的表达及意义

雒伟伟¹, 邢沈阳¹, 赵志辉¹, 张西臣²

1. 吉林大学畜牧兽医学院动物遗传育种与繁殖学系|吉林　长春 130062;2.吉林大学畜牧兽医学院预防兽医学系|吉林　长春 130062

收稿日期:2009-12-30 出版日期:2010-07-28 发布日期:2010-07-28
通讯作者: 邢沈阳（Tel:0431-87836177，E-mail: xingsy6177@yahoo.com.cn)；张西臣（Tel:0431-87836155，E-mail: zhangxic@public.cc.jl.cn） E-mail:xingsy6177@yahoo.com.cn；zhangxic@public.cc.jl.cn
作者简介:雒伟伟（1982-）|男|山西省临县人|在读理学博士|主要从事转基因动物研究。
基金资助:
国家重大基础研究计划资助课题（2006CB910505）

Expression of FOXD1 in human breast cancer tissues and significance

LUO Wei-Wei¹, XING Shen-Yang¹, ZHAO Zhi-Hui¹, ZHANG Xi-Chen²

1．Department of Animal Genetics and Reproduction,College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China;2. Department of Animal Preventive Veterinary Medicine,College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China

Received:2009-12-30 Online:2010-07-28 Published:2010-07-28

摘要/Abstract

摘要：

目的:检测转录因子FOXD1在人浸润性乳腺癌和乳腺癌癌旁组织中的表达量变化，为进一步阐明FOXD1在人乳腺癌发生、发展中的意义提供依据。方法：应用冰冻切片和HE染色方法将113例乳腺癌标本分为浸润性乳腺癌和乳腺癌癌旁组织；选取4例浸润性乳腺癌标本和4例乳腺癌癌旁组织标本，应用qRT-PCR技术和Western blotting方法测定FOXD1在转录水平和翻译水平的相对表达量。结果:按组织分类学标准，113例乳腺癌病例标本分为乳腺癌癌旁组织36例、浸润性乳腺癌组织77例。qRT-PCR检测表明：与乳腺癌癌旁组织比较，浸润性乳腺癌组织中FOXD1的相对表达量显著升高（P<0.01）。Western blotting分析表明：浸润性乳腺癌组织中FOXD1的相对表达量明显高于乳腺癌癌旁组织中的相对表达量（P<0.05）。结论:FOXD1在浸润性乳腺癌组织中的表达量明显高于乳腺癌癌旁组织，提示FOXD1表达水平与乳腺癌发生和发展有关。

关键词: FOXD1；乳腺肿瘤；Western blotting；实时荧光定量PCR

Abstract:

Objective
To detect the changes of FOXD1 expression in invasive breast cancer tissues and tissues surrounding breast cancer,and provide basis for further study on the functions of FOXD1 in the occurrence and development of breast cancer. Methods 113 samples were classified into invasive breast cancer tissue and tissues surrounding breast cancer by frozen section and HE staining.Then the expression levels in four samples of invasive breast cancer tissues and another four samples of tissues surrounding breast cancer were examined by qRT-PCR and Western blotting respectively.Results 113 samples were classified into 36 cases of invasive breast cancer tissues and 77 cases of tissues surrounding breast cancer according to standards of pathohistology.The qRT-PCR result showed that the FOXD1 expression in invasive breast cancer tissues was increased significantly compared with tissues surrounding breast cancer (P<0.01).The result of Western blotting also demonstrated that the FOXD1 expression in invasive breast cancer tissues was higher than that in tissues surrounding breast cancer (P<0.05).Conclusion The expression of FOXD1 in breast cancer tissue is significantly higher than that in tissues surrounding breast cancer. The result indicates that the expression level of FOXD1 is related to the occurrence and development of breast cancer.

Key words: FOXD1;breast neoplasms;Western blotting;qRT-PCR

中图分类号:

R737.9

雒伟伟, 邢沈阳, 赵志辉, 张西臣. 转录因子FOXD1在乳腺癌组织中的表达及意义[J]. J4, 2010, 36(4): 703-707.

LUO Wei-Wei, XING Shen-Yang, ZHAO Zhi-Hui, ZHANG Xi-Chen.
Expression of FOXD1 in human breast cancer tissues and significance[J]. J4, 2010, 36(4): 703-707.

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