HLA-DRB1等位基因与吉林地区汉族人群慢性乙型病毒性肝炎的关联性分析

J4 ›› 2010, Vol. 36 ›› Issue (4): 741-744.

HLA-DRB1等位基因与吉林地区汉族人群慢性乙型病毒性肝炎的关联性分析

韩瑜¹, 马宁², 焦立新¹, 林乾飞¹, 姜振宇²

1.吉林省血液中心输血研究所|吉林长春 130033；2.吉林大学第一医院风湿病科|吉林长春 130021

收稿日期:2010-01-05 出版日期:2010-07-28 发布日期:2010-07-28
通讯作者: 姜振宇(Tel：0431-84808382，E-mail：jlujzy@yahoo.com.cn) E-mail:jlujzy@yahoo.com.cn
作者简介:韩瑜（1976-）|男|吉林省长春市人|主管技师|医学硕士|主要从事人类白细胞抗原遗传方面的研究。
基金资助:
吉林省科技厅科研基金资助课题(20090454)

Relevance analysis on HLA- DR1 alleles and chronic hepatitis B of Han population in Jilin province

HAN Yu¹, MA Ning², JIAO Li-Xin¹, LIN Gan-Fei¹, JIANG Zhen-Yu²

1. |Institute of Blood Transfusion|Jilin Blood Center |Changchun 130033|China；2. Department of Rheumatology|First Hospital|Jilin University|Changchun 130021|China

Received:2010-01-05 Online:2010-07-28 Published:2010-07-28

摘要/Abstract

摘要：

目的：探讨HLA-DRB1基因多态性与吉林地区汉族人群慢性乙型肝炎病毒(HBV)感染的相关关系，为慢性乙型病毒性肝炎的治疗和预防提供新思路。方法：采用聚合酶链反应-序列特异性寡核苷酸探针技术(PCR-SSOP)对398例汉族慢性乙型病毒性肝炎患者及838名汉族健康献血者（对照组）进行HLA-DRB1等位基因分型比较。结果：慢性乙型肝炎组HLA-DRB1*03、09等位基因表达频率分别为6.28%和19.60%，明显高于健康对照组（3.58%和12.17%；P=0.031，OR=1.805；P=0.001，OR=1.759)；慢性乙型肝炎组HLA-DRB1*04、10和13等位基因表达频率分别为6.28%、0.50%和3.27%，明显低于健康对照组（11.34%、2.39%和5.97%；P=0.005，OR=0.524；P=0.019，OR=0.207；P=0.044，OR=0.523）；而所检测HLA-DRB1座位的其他等位基因表达频率与健康对照组比较差异无显著性(P>0.05)。结论：HLA-DRB1*03、09可能是吉林地区汉族慢性乙型病毒性肝炎患者易感基因，HLA-DRB1*04、10、13可能是保护基因。

关键词: 慢性乙型病毒性肝炎；人类白细胞抗原；PCR-SSOP ；汉族

Abstract:

Abstract:Objective To explore the correlation between the HLA-DRB1 alleles and the chronic hepatitis B of the Han population in Jilin province，and provide a new way for treatment and prevention of chronic hepatitis B．Methods HLA-DRB1 alleles in 398 patients with chronic hepatitis B and 838 healthy controls were analyzed by using the polymerase chain reaction-sequence specific oligonucleotide probe (PCR-SSOP) technique.Results The frequencies of HLA-DRB1*03，09 alleles in chronic hepatitis B group were higher than those in healthy control group(6.28％ vs 3.58％，P=0.031，OR=1.805； 19.60% vs 12.17%，P=0.001，OR=1.759)．The frequencies of HLA-DRB1*04，10 and 13 alleles in chronic hepatitis B group were lower than those in healthy control group (6.28％ vs 11.34％，P=0.005，OR=0.524；0.50% vs 2.39%，P=0.019，OR=0.207；3.27％ vs 5.97％，P=0.044，OR=0.523)．There were no significant differences in the frequencies of the other HLA-DRB1 alleles between chronic hepatitis B group and healthy control group(P>0.05).Conclusion The HLA-DRB1*03，09 alleles may be susceptible genes for chronic hepatitis B，and HLA-DRB1*04，10，13 alleles may be protecting gene for chronic hepatitis B of Han population in Jilin province．

Key words: chronic hepatitis B；human leukocyte antigen-DRB1；PCR-SSOP；Han nationality

中图分类号:

R512.6

韩瑜, 马宁, 焦立新, 林乾飞, 姜振宇. HLA-DRB1等位基因与吉林地区汉族人群慢性乙型病毒性肝炎的关联性分析[J]. J4, 2010, 36(4): 741-744.

HAN Yu, MA Ning, JIAO Li-Xin, LIN Gan-Fei, JIANG Zhen-Yu. Relevance analysis on HLA- DR1 alleles and chronic hepatitis B of Han population in Jilin province[J]. J4, 2010, 36(4): 741-744.