电离辐射对Jurkat细胞γ-H2AX蛋白表达的影响

J4 ›› 2010, Vol. 36 ›› Issue (5): 895-899.

电离辐射对Jurkat细胞γ-H2AX蛋白表达的影响

倪冠英^1,2, 何淑梅³, 董娟聪¹, 金顺子¹

1.吉林大学公共卫生学院卫生部放射生物学重点实验室|吉林长春 130021；2.吉林省肿瘤医院核医学科|吉林长春 130012；3.吉林大学第一医院干部病房|吉林长春 130021

收稿日期:2010-04-10 出版日期:2010-09-28 发布日期:2010-09-28
通讯作者: 金顺子（Tel:0431-85619443,E-mail:shunzij@yahoo.com.cn） E-mail:shunzij@yahoo.com.cn
作者简介:倪冠英（1983－）|男|吉林省长春市人|医学硕士|主要从事开发新型辐射生物剂量计的研究。
基金资助:
卫生部公益性行业科研专项基金资助课题（200802018）

Effects of ionizing radiation on expression of γ-H2AXin Jurkat cell lines

NI Guan-Ying^1,2, HE Chu-Mei³, DONG Juan-Cong¹, JIN Shun-Zi¹

1.Key Laboratory of |Radiobiology, Ministry of Health,School of Public Health,Jilin University,Changchun 130021,China|2.Department of Neclear Medicine,Jilin Tumor Hospital,Changchun 130012,China; 3.Department of Cadre Ward,First Hospital, Jilin University,Changchun 130021,China

Received:2010-04-10 Online:2010-09-28 Published:2010-09-28

摘要/Abstract

摘要：

目的：探讨电离辐射诱导Jurkat细胞γ-H2AX蛋白表达的影响。方法：采用流式细胞术（FCM）分别检测2.0 Gy X线照射后不同时间点（0、0.5、1.0、4.0、8.0、16.0及24.0 h）和0.5、1.0、2.0、4.0及6.0 Gy不同剂量X线照射后Jurkat细胞γ-H2AX表达变化，并于量效实验同时进行Jurkat细胞凋亡率检测。结果：与假照组比较，2.0 Gy X线照射后Jurkat细胞γ-H2AX表达水平于1.0 h内达最大值，1.0 h后开始呈时间依赖性下降，16.0 h仍高于假照水平（P<0.01），24.0 h降至与对照无明显差异；不同剂量X线照射后1.0 h，Jurkat细胞γ-H2AX的表达水平在0.5 Gy以内没有明显变化，0.5 Gy以后呈剂量依赖性升高，4.0 Gy时达最高水平（P<0.01），6.0 Gy时表达略下降；不同剂量X线照射后8.0 h，γ-H2AX表达在0～4.0 Gy范围内随剂量增大而逐渐升高，4.0 Gy时达到最高水平（P<0.01），6.0 Gy时表达有所下降。结论：X射线能诱导Jurkat细胞γ-H2AX表达增高，在一定范围内存在时间和剂量依赖关系。

关键词: 电离辐射；蛋白表达；γ-H2AX；Jurkat细胞

Abstract:

Abstract:Objective
To investigate the effects of ionizing radiation on the expression of γ-H2AX in Jurkat cell lines. Methods The changes of the expressions of γ-H2AX in Jurkat cell lines after X-irradiation with different doses(0.5,1.0,2.0,4.0,and 6.0 Gy) and after irradiation with 2.0 Gy X-rays for different time(0,0.5,1.0,4.0,8.0,16.0,and 24 h) were analyzed by flow cytometry (FCM),and the apoptotic rate of Jurkat cell lines was detected during dose-effect experiment. Results In time-effect experiment,the results showed that the expression of γ-H2AX reached to the peak within 1 h,followed by a time-dependent decrease,kept expressing at 16.0 h,and had no significant difference with control at 24.0 h. In dose-effect experiment,it was demonstrated that the expressions of γ-H2AX showed a dose-dependent increase in the range of 0.5-4.0 Gy at 1.0 h after exposed to X-rays,and dropped at the does of 6.0 Gy;at 8.0 h after exposure to those doses above,the express levels of γ-H2AX enhanced linearly with the dose increased in the range of 0-4.0 Gy,showing a clear dose-dependent manner(P<0.01). Conclusion The γ-H2AX expression could be increased by X-irradiation in time-dependent and dose-dependent manners.

Key words: irradiation;radiation;protein expression;γ-H2AX;Jurkat cells

中图分类号:

R811.5

倪冠英, 何淑梅, 董娟聪, 金顺子. 电离辐射对Jurkat细胞γ-H2AX蛋白表达的影响[J]. J4, 2010, 36(5): 895-899.

NI Guan-Ying, HE Chu-Mei, DONG Juan-Cong, JIN Shun-Zi. Effects of ionizing radiation on expression of γ-H2AXin Jurkat cell lines[J]. J4, 2010, 36(5): 895-899.