关键词: 人参皂苷Rg1；髓鞘碱性蛋白；白质纤维损伤

Abstract:

Abstract:Objective To study the influence of ginsenoside Rg1 on injury of white matter and illuminate the protective effect of ginsenoside Rg1 and its mechanism after cerebral ischemia. Methods Male ICR mice were pre-treated with ginsenoside Rg1 (50 mg?kg-1) by intragastric (i.g.) injection once a day for 7 days. One hour after the last injection bilateral common carotid arteries were occluded for 60 min. The mice were divided into four groups:sham group(Sal-Sham),drug control group (Gs-Sham),ischemia group (Sal-Isc) and drug-protective group (Gs -Isc).The injury of white matter in dentate gyrus of hippocampus after global cerebral ischemia was detected with immunohistochemical  staining.The MDA protein expression after           cerebral ischemia was determined with Western blotting method.Results Compared with sham group,the MBP positive cells  in ischemia group were decreased（P<0.05）; compared with ischemia group,the MBP positive cells  in drug-protective group were increased（P<0.05）;compared with sham group,the MDA protein  in ischemia group was increased（P<0.05）;compared with ischemia group,the MDA protein  in drug-protective group was decreased（P<0.05）.Conclusion Ginsenoside Rg1 can attenuate the injury of white matter after cerebral ischemia and its mechanism might be associated with decreasing the MDA protein expression.

Key words: ginsenoside Rg1；myelin basic protein; , white matter injury