黄连素对2型糖尿病大鼠主动脉内皮功能和形态的影响

J4 ›› 2010, Vol. 36 ›› Issue (6): 1016-1020.

黄连素对2型糖尿病大鼠主动脉内皮功能和形态的影响

张海英¹, 王春梅^2,3, 张明², 吕晓艳², 王云晶², 陈立², 杜红伟⁴

(1.吉林大学基础医学院病理解剖学教研室|吉林长春 130021；2.吉林大学基础医学院药理学教研室|吉林长春 130021；3.北华大学药学院药理学教研室|吉林吉林 132013；4.吉林大学第一医院小儿内分泌科|吉林长春 130021)

收稿日期:2010-03-25 出版日期:2010-11-28 发布日期:2010-11-28
通讯作者: 陈立 E-mail:陈立（Tel: 0431-85619799,E-mail:zhouweichen
作者简介:张海英(1975-) |女|吉林省舒兰县人|实验师|医学硕士|主要从事肿瘤生物学研究。
基金资助:
国家自然科学基金资助课题（30772604）;吉林省科技厅高技术产业发展项目资助课题（2009）

Effects of berberine on aortic endothelial function and morphous |in type 2 diabetic rats

ZHANG Hai-Yang¹, WANG Chun-Mei^2,3, ZHANG Meng², Lu Xiao-Yan², WANG Yun-Jing², CHEN Li², DU Hong-Wei⁴

(1.Department of Pathology School Basic Medical Sciences,Jilin University,Changchun 130021,China;2.Department of Pharmacology| School of Basic Medical Sciences,Jilin University,Changchun 130021,China;3.Department of Pharmacology,School of Pharmacy,Beihua University,Jilin 132013,China;4.Department of Pediatrics, First Hospital,Jilin University,Changchun 130021,China)

Received:2010-03-25 Online:2010-11-28 Published:2010-11-28

摘要/Abstract

摘要：

目的：通过观察黄连素对2型糖尿病大鼠血管内皮功能的影响，探讨黄连素对2型糖尿病血管并发症的防治意义及其机制。方法：采用小剂量链脲佐菌腹腔注射加高脂饲料喂养的方法建立2型糖尿病大鼠模型，将模型动物随机分为模型组及黄连素治疗组，另设正常对照组以及正常黄连素组。药物干预治疗8 周后，处理动物，检测各组动物血清中空腹血糖（FBG）、总胆固醇（TC）、甘油三酯（TG）及空腹血清胰岛素（FINS）的水平；通过离体实验方法观察离体胸主动脉条的张力反应；HE染色观察胸主动脉形态学改变。结果：与模型组比较，黄连素治疗组大鼠FBG、TC及TG 水平明显下降，胰岛素敏感指数（ISI）升高 ( P<0.05)；黄连素治疗组大鼠乙酰胆碱（ACh）引起的内皮依赖性血管舒张反应明显改善，而硝普钠（SNP）诱导的内皮非依赖性舒张反应各组间比较差异无显著性(P>0.05)；黄连素治疗组大鼠糖尿病引起的血管内皮病变明显减轻；与正常对照组比较，正常大鼠给予黄连素灌胃，上述指标未出现明显改变。结论：黄连素对2 型糖尿病早期出现的血管内皮损伤有良好的保护作用，机制可能与其降血糖、调节血脂、改善胰岛素抵抗及内皮依赖性的血管舒张反应等作用有关。

关键词: 黄连素；糖尿病, 实验性；血管内皮；内皮功能障碍

Abstract:

Abstract:Objective To investigate the effects of berberine on vascular endothelial function in type 2 diabetic rats and explore the prophylactic and therapeutic significance and pharmacological mechanism of berberine in type 2 diabetic vascular complications. Methods Wistar rats were randomly divided into four groups: diabetic group,control group,diabetic rats treated with berberine (100 mg·kg-1) group,and control rats treated with berberine group. The serum fasting blood glucose(FBG),insulin,total cholesterol(TC),triglyceride(TG) and fasting insulin(FINS) levels were tested. Acetylcholine (ACh)-induced endothelium-dependent relaxation and sodium nitroprusside
-induced endothelium-independent relaxation were measured in aortas for estimating endothelial function. In addition,the histopathology measurement was performed with hematoxylin and eosin staining. Results Compared with diabetic group,the FBG,TC and TG levels in diabetic rats treated with berberine were significantly decreased,meanwhile the insulin sensitivity index(ISI) was increased. The Ach-induced endothelium-dependent relaxation in the aorta from diabetic rats was significantly improved in berberine treatment group,while sodium nitroprusside (SNP)-induced endothelium-independent relaxation showed no difference among four groups. The histopathological results showed that berberine attenuated the damage of the aortic endothelium in diabetic rats. However there was no difference in these indexes between control group and berberine treated control group. Conclusion Berberine restores diabetic endothelial dysfunction through decreasing the FBS and blood lipid,improving insulin resistant and enhancing NO bioavailability.

Key words: berberine;diabetes,mellitus experimental;vascular endothelium;endothelial dysfunction

中图分类号:

R587.1

张海英, 王春梅, 张明, 吕晓艳, 王云晶, 陈立, 杜红伟. 黄连素对2型糖尿病大鼠主动脉内皮功能和形态的影响[J]. J4, 2010, 36(6): 1016-1020.

ZHANG Hai-Yang, WANG Chun-Mei, ZHANG Meng, Lu Xiao-Yan, WANG Yun-Jing, CHEN Li, DU Hong-Wei. Effects of berberine on aortic endothelial function and morphous |in type 2 diabetic rats[J]. J4, 2010, 36(6): 1016-1020.

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