p53在异丙肾上腺素所致心肌纤维化大鼠心肌组织中的表达及意义

J4 ›› 2010, Vol. 36 ›› Issue (6): 1064-1066.

p53在异丙肾上腺素所致心肌纤维化大鼠心肌组织中的表达及意义

苗春生¹|高海成¹|李相军¹|尹俐¹|李蕴潜²|李才¹

1. 吉林大学药学院病理与毒理学教研室|吉林长春 130021；2. 吉林大学第一医院神经外科|吉林长春 130021

收稿日期:2010-06-07 出版日期:2010-11-28 发布日期:2010-11-28
通讯作者: 李才（Tel：0431-85619702，E-mail：lic@jlu.edu.cn） E-mail:lic@jlu.edu.cn
作者简介:苗春生（1964-）|男|吉林省桦甸市人|主管技师|主要从事实验病理学研究。
基金资助:
吉林省科技厅科技发展计划重大项目资助课题（20020503-4）

Expression of p53 in myocardium tissue of myocardial fibrosis rats induced with isoproterenol |and its significance

MIAO Chun-sheng¹,GAO Hai-cheng¹,LI Xiang-jun¹,YIN Li¹,LI Yun-qian²,LI Cai¹

1. Department of Experimental Pharmacology and Toxicology,School of Pharmacy, Jilin University,Changchun 130021,China;2. Department of Neurosurgery,First Hospital, Jilin University,Changchun 130021,China

Received:2010-06-07 Online:2010-11-28 Published:2010-11-28

摘要/Abstract

摘要：

目的：探讨p53在异丙肾上腺素（Iso）所致心肌纤维化大鼠心肌组织中的表达及意义，阐明其在心肌纤维化中的作用。方法：25只大鼠随机分为5组，正常对照组及注射Iso后12 h、24 h、1周和3周组。除正常对照组外，其他各组皮下多点注射Iso（15 mg·kg^-1）复制大鼠心肌损伤模型。采用免疫组织化学染色及RT-PCR分别检测心肌p53蛋白及基因表达。结果：免疫组织化学染色显示，注射Iso后12和24 h心肌P53蛋白表达水平明显高于正常对照组（P<0.05），注射1和3周后p53蛋白表达增加更明显（P<0.01）。RT-PCR结果显示，与正常对照组比较，注射Iso后12 h心肌组织中p53 mRNA表达明显增高（P<0.05），注射Iso后24 h p53 mRNA表达水平达到最高（P<0.01），而注射Iso后1周心肌p53 mRNA表达较注射后24 h有所下降，但仍高于正常对照组(P<0.05)。结论：P53蛋白在大鼠损伤心肌中呈持续高表达，p53基因表达上调，提示p53在心肌纤维化形成过程中起一定作用。

关键词: p53基因；异丙肾上腺素；心肌纤维化；细胞凋亡

Abstract:

To investigate the expression of p53 in myocardium tissue of ispproterenol (Iso)[KG-*3]-induced myocardial fibrosis rats and its signigicance and clarify the effect in inyocardial of fibrosis. Methods 25 male Wistar rats were randomly divided into five groups: control group，model groups(12 h,24 h,1 week and 3 weeks)(n=5). Except control group,all the rats in the other groups were treated with Iso to bulit rat myocardial injury models(15 mg·kg^-1).The expressions of P53 protein and p53 mRNA were detected with immunohistochemical staining and RT-PCR.Results The immunohistochemical results showed that the P53 protein expressions were increased 12 and 24 h after Iso injection compared with control group（P<0.05）and the protein expressions were obviously increased 1 week and 3 weeks after Iso injection（P<0.01）.The RT-PCR results showed that the p53 mRNA was obviously increased at 12 h (P<0.05),arrived a peak at 24 h (P<0.01)　and was gradually decreased at 1 week,but still higher than normal level (P<0.05). Conclusion The P53 protein expression is increased in rat myocardium tissue and the expression of p53 gene is up-regulated,suggesting that p53 has an important role in rat myocardial fibrosis.

Key words: p53 gene;isoproterenol;myocardial fibrosis;apoptosis

中图分类号:

R542.21

苗春生, 高海成, 李相军, 尹俐, 李蕴潜, 李才. p53在异丙肾上腺素所致心肌纤维化大鼠心肌组织中的表达及意义[J]. J4, 2010, 36(6): 1064-1066.

MIAO Chun-Sheng, GAO Hai-Cheng, LI Xiang-Jun, YIN Li, LI Yun-Qian, LI Cai. Expression of p53 in myocardium tissue of myocardial fibrosis rats induced with isoproterenol |and its significance[J]. J4, 2010, 36(6): 1064-1066.