S180腹水癌小鼠肿瘤浸润淋巴细胞中CD4+CD25+Treg细胞在肿瘤逃逸中的作用

J4 ›› 2010, Vol. 36 ›› Issue (6): 1094-1097.

S180腹水癌小鼠肿瘤浸润淋巴细胞中CD4+CD25+Treg细胞在肿瘤逃逸中的作用

历春^1,2, 李欣¹, 贾婷¹, 孙际童¹, 付海英¹

1.吉林大学基础医学院免疫学系|吉林长春 130021； 2.北华大学基础医学院病理学教研室|吉林吉林132013

收稿日期:2010-05-31 出版日期:2010-11-28 发布日期:2010-11-28
通讯作者: 付海英(Tel: 0431-85619476，E-mail: fuhaiying612@yahoo.com.cn) E-mail:fuhaiying612@yahoo.com.cn
作者简介:历春（1972-）|女|吉林省吉林市人|讲师|在读医学博士|主要从事肿瘤免疫耐受研究。
基金资助:
吉林大学基本科研业务费科学前沿与交叉学科创新项目资助课题（200903007）

Function of CD4+CD25+Treg cells of tumor infiltrating lymphocytes in S180 ascites carcinoma mice in tumor immune escape

LI Chun^1,2, LI Xin¹, JIA Ting¹, SUN Ji-Tong¹, FU Hai-Ying¹

1. Department of Immunology,School of Basic Medical Sciences,Jilin University,Changchun 130021,China；2. Department of Pathology,Basic Medical College,Beihua University,Jilin 132013,China

Received:2010-05-31 Online:2010-11-28 Published:2010-11-28

摘要/Abstract

摘要：

目的：研究肿瘤浸润淋巴细胞（TIL）中CD4⁺CD25⁺Treg细胞及免疫监视功能的变化，探讨CD4⁺CD25⁺Treg 细胞在肿瘤免疫逃逸中的作用。方法：建立小鼠S180腹水癌模型，采集S180腹水癌小鼠TIL、脾脏细胞及对照组小鼠脾脏细胞，采用流式细胞术检测CD4⁺CD25⁺Treg细胞、CD8⁺T细胞、NK细胞的数量及NKG2D的表达水平，采用RT-PCR方法检测Foxp3 mRNA的表达水平，采用乳酸脱氢酶(LDH) 释放法检测CD8⁺T细胞的杀伤功能。结果：与对照组小鼠比较，S180腹水癌小鼠脾脏、TIL中CD4⁺CD25⁺Treg细胞数量和Foxp3 mRNA表达均明显增高(P<0.05)，并且肿瘤组小鼠TIL中CD4⁺CD25⁺Treg细胞数量和Foxp3 mRNA表达较肿瘤组小鼠脾脏均明显增高(P<0.05)；TIL中CD8⁺T细胞数量明显增高(P<0.05)，其杀伤功能却有所下降；TIL中NK细胞数量及NKG2D表达均明显增高 (P<0.05)。结论：肿瘤局部CD4⁺CD25⁺Treg细胞数量增加、功能增强，可能是肿瘤逃避免疫监视的机制之一。

关键词: CD4+CD25+Treg细胞；Foxp3；肿瘤浸润淋巴细胞；肿瘤免疫

Abstract:

Objective To study the changes of CD4⁺CD25⁺Treg cells and immunesurveillance function in tumor infiltrating lymphocytes(TIL) and investigate the relationship between CD4⁺CD25⁺Treg cells and tumor immune escape. Methods TIL and spleen cells of the S180 ascites carcinoma mice and spleen cells of the control ones were used for the following detection: the quantities of CD4⁺CD25⁺Treg cells,CD8⁺T cells,NK cells and NKG2D were detected by flow cytometry;the expression level of Foxp3 mRNA was detected by RT-PCR;the killing function of CD8⁺T cells was detected by lactate dehydrogenase release assay. Results Compared with control mice，the quantity of CD4⁺CD25⁺Treg cells and the expression of Foxp3 mRNA were significantly increased in the TIL and spleen cells of the tumor mice (P<0.05);furthermore,the quantity of CD4⁺CD25⁺Treg cells and the expression of Foxp3 mRNA in TIL were both higher than those in spleen cells of tumor mice;the number of CD8⁺T cells in TIL was significantly increased(P<0.05),while the killing function of CD8⁺T cells in TIL was decreased;the number of NK cells and the expression of NKG2D in TIL were significantly increased (P<0.05). Conclusion The quantity and function of CD4⁺CD25⁺Treg cells are significantly increased in location of tumor which might be one of the mechanisms of tumor escaping from immune surveillance.

Key words: CD4+CD25+Treg cells;Foxp3;tumor infiltrating lymphocytes;tumor immunity

中图分类号:

R730.3

历春, 李欣, 贾婷, 孙际童, 付海英. S180腹水癌小鼠肿瘤浸润淋巴细胞中CD4+CD25+Treg细胞在肿瘤逃逸中的作用[J]. J4, 2010, 36(6): 1094-1097.

LI Chun, LI Xin, JIA Ting, SUN Ji-Tong, FU Hai-Ying. Function of CD4+CD25+Treg cells of tumor infiltrating lymphocytes in S180 ascites carcinoma mice in tumor immune escape[J]. J4, 2010, 36(6): 1094-1097.