关键词: RNA干扰；高迁移率族核小体结合域5；肺肿瘤；细胞增殖；细胞周期

Abstract:

Abstract:ObjectiveTo explore the effect of high-mobility group mucleosome binding domain 5(HMGN5) by RNAi on proliferation and cell cycle of lung cancer cell line H1299，and provide a theoretical basis for lung cancer targeted therapy. Methods SiRNA targeting HMGN5 gene was cloned into lentivirus vector.SiRNA-HMGN5 lentivirus particles were infected into H1299 cells in order to silence the expression of HMGN5. Negative control group and RNAi group were set up. The interfering efficiencies of the plasmids on HMGN5 gene were detected at the mRNA and protein levels by Real-time PCR and Western blotting. The proliferation  of H1299 was analyzed by MTT and BrdU assay. The cell cycle was detected by flow cytometry. Results Compared with negative control group,the expression of HMGN5 mRNA in RNAi group was down-regulated by 50.7%(P< 0.05);the protein level was significantly deceased(P<0.05). Compared
with negative control group,the proliferation rate of H1299 cells was deceased by MTT assay. BrdU assay results showed that the cell proliferation rate of  H1299 in  RNAi group (37.8%) was significantly lower than that in negative control group(55.0%)(P< 0.05).The percentage of cells at G1 phase（54.6%±0.9%） in  RNAi group was inceased compared with negative control group（46.5%±0.4%）(P< 0.05). Conclusion Silencing HMGN5 gene can inhibit the proliferation of H1299 cells.It shows that HMGN5 plays a role in the development of lung cancer and it may be beneficial in finding a new therapy for lung cancer.

Key words: RNA interference;high-mobility group nucleosome binding domain 5;lung neoplasms;cell proliferation;cell cycle