系统性红斑狼疮患者血清增殖诱导配体水平的检测及其临床意义

J4 ›› 2011, Vol. 37 ›› Issue (1): 105-108.

系统性红斑狼疮患者血清增殖诱导配体水平的检测及其临床意义

赵海燕, 张晓莉, 郭韵, 蒋莉, 肖卫国

1.中国医科大学附属盛京医院风湿免疫科|辽宁沈阳 110004；2.中国医科大学附属第一医院风湿免疫科|辽宁沈阳 110001

收稿日期:2010-01-11 出版日期:2011-01-28 发布日期:2010-01-28
通讯作者: 肖卫国（Tel:024-83282550,E-mail:xonet5670@163.com） E-mail:xonet5670@163.com
作者简介:赵海燕（1984－）|女|满族|辽宁省海城市人|医师|医学硕士|主要从事系统性红斑痕疮及类风湿性关节炎的基础与临床研究。
基金资助:
辽宁省科技厅科学计划项目资助课题（2007225013）

Determination of a proliferation-inducing ligand |in patients with systemic lupus erythematosus and its clinical significance

ZHAO Hai-Yan, ZHANG Xiao-Li, GUO Yun, JIANG Li, XIAO Wei-Guo

1.Department of Rheumatology,Shengjing Hospital,China Medical University,Shenyang 110004,China；2.Department of Rheumatology,First Hospital,China Medical University,Shenyang 110001,China

Received:2010-01-11 Online:2011-01-28 Published:2010-01-28

摘要/Abstract

摘要：

目的：检测系统性红斑狼疮(SLE)和类风湿性关节炎(RA)患者血清中增殖诱导配体（APRIL）的水平，探讨APRIL水平与SLE患者临床指标的相关性及APRIL在SLE发病中的作用。方法：采用ELISA法检测SLE患者组(48例)、RA患者组(16例)和正常对照组(16例)血清APRIL水平，并与SLE活动指数（SLEDAI）及实验室指标进行对比分析。结果：SLE组和RA组血清APRIL水平高于正常对照组（P<0.01），SLE组高于RA组（P<0.01）。SLE组中抗Sm抗体阳性患者APRIL水平高
于阴性患者（ P<0.05）。抗U1-RNP抗体阳性患者APRIL水平高于阴性患者（ P<0.05），抗SSA /SSB抗体、抗ACL抗体、抗ds-DNA抗体阳性和阴性患者APRIL水平差异无统计学意义。SLE患者血清APRIL水平与其补体C3、C4呈负相关关系（r1=－0.819，P<0.01；r2=－0.549，P<0.01），与SLEDAI评分、免疫球蛋白、抗核抗体谱等免疫学指标无相关性。结论：APRIL在SLE患者中特异性升高，可能在狼疮发病中起重要作用;血清APRIL水平可能与SLE患者疾病活动程度有关联，但尚不能确定是否可作为疾病活动性指标。

关键词: 系统性红斑狼疮；类风湿性关节炎；增殖诱导配体；酶联免疫吸附试验

Abstract:

Objective
To determine the serum levels of a proliferation-inducing ligand(APRIL) in patients with systemic lupus erythematosus (SLE) and rheumatoid arthritis(RA) and analyze its correlations with immunological parameters and its roles in the development of SLE.Methods The levels of APRIL in 48 patients with SLE,16 patients with RA and 16 healthy controls were determined by enzyme linked immunosorbent assay (ELISA),its correlations with SLEDAI and immunological parameters were analyzed.Results The serum levels of APRIL in SLE and RA patients were significantly higher than that in healthy controls （P<0.01）,and the serum level of APRIL in SLE patients were significantly higher than that in RA patients（P<0.01）. The serum level of APRIL in SLE patients with positive anti-Sm antibody was significantly higher than that in the negative ones（P<0.05）.The serum level of APRIL in SLE patients with positive anti-U1RNP antibody was significantly higher than that in the negative ones（P<0.05）.While the serum level of APRIL in SLE patients didn’t have significant differences between the patients with positive anti-SSA /SSB antibody,anti-ACL antibody,anti-dsDNA antibody and the negative ones.The serum level of APRIL in SLE patients was negatively correlated with the complements C3 and C4 （r1=－0.819，P1＜0.01；r2=－0.549，P2＜0.01）,but not significantly correlated with SLEDAI and other immunological parameters.Conclusion The serum level of APRIL in SLE patients is specially increased,it may have an important effect on the development of SLE and may be correlated with the activity of SLE .

Key words: systemic lupus erythematosus;rheumatoid arthritis;a proliferation-inducing ligand;enzyme linked immunosorbent assay

中图分类号:

R593.24

赵海燕, 张晓莉, 郭韵, 蒋莉, 肖卫国. 系统性红斑狼疮患者血清增殖诱导配体水平的检测及其临床意义[J]. J4, 2011, 37(1): 105-108.

ZHAO Hai-Yan, ZHANG Xiao-Li, GUO Yun, JIANG Li, XIAO Wei-Guo. Determination of a proliferation-inducing ligand |in patients with systemic lupus erythematosus and its clinical significance[J]. J4, 2011, 37(1): 105-108.