雄激素受体基因 E211 G>A多态性与汉族女性青春期后痤疮的关联性分析

J4 ›› 2011, Vol. 37 ›› Issue (1): 113-116.

雄激素受体基因 E211 G>A多态性与汉族女性青春期后痤疮的关联性分析

田黎明¹, 谢红付¹, 杨婷², 王玮蓁³, 李吉¹

1.中南大学湘雅医院皮肤科|湖南长沙 410008;2.昆明医学院第一附属医院皮肤科|云南昆明 650032;3.华中科技大学武汉第一医院皮肤科|湖北武汉 430022

收稿日期:2010-07-22 出版日期:2011-01-28 发布日期:2011-01-28
通讯作者: 田黎明（Tel:0731-84327472,E-mail:jolly180@yahoo.cn） E-mail:jolly180@yahoo.cn

Relevance analysis on E211 G>A androgen receptor gene polymorphism and 　 female postadolescent acne in Han population

TIAN Li-Ming¹, XIE Hong-Fu¹, YANG Ting², WANG Wei-Zhen³, LI Ji¹

Received:2010-07-22 Online:2011-01-28 Published:2011-01-28

摘要/Abstract

摘要：

目的：探讨雄激素受体(AR)基因E211 G>A单核苷酸多态性与汉族女性青春期后痤疮的关联性，为汉族女性青春期后痤疮发病遗传学研究奠定基础。　方法：将女性青春期后痤疮患者79例和正常女性志愿者80例分为女性青春期后痤疮组和女性正常对照组，采用PCR技术扩增出AR-E211 G>A基因多态位点的片段,用限制性内切酶StuⅠ进行酶切,产物在2%琼脂糖凝胶上电泳,确定G>A基因的3种基因型,即GG、GA、AA，观察两组基因型频数和等位基因型频数分布的差异。结果：女性青春期后痤疮组GA+AA基因型频数低于对照组（P=0.028,OR=0.937,95% CI=0.885～0.992）。女性青春期后痤疮组A等位基因频数低于对照组（P=0.029,OR=0.968,95% CI=0.941～0.996）。结论:AR-E211 A等位基因的存在使青春期后女性患痤疮的风险性明显降低。

关键词: 雄激素受体；基因多态性；女性青春期后痤疮；汉族；遗传学

Abstract:

Abstract:Objective
To explore the correlation between the E211 G>A androgen receptor(AR) gene polymorphism and female postadolescent acne of Han Chinese,and lay the foundation for genetic studies of female postadolescent acne of Han Chinese. Methods The female postadolescent acne patients(n=79) and the healthy people(n=80) were individed into female postadolescent acne group and control group. The polymorphism was investigated by PCR using DNA from peripheral blood lymphocytes. The transition(G>A) in the risk allele(A) produced a new recognition site for the estriction endonuclease StuⅠ. Three genotypes of AR-E211 G>A gene (GG,GA,AA) were determined,and the differences in the frequencies of the genotype and the allele between female postadolescent acne group and control group were observed. Results There was a significant difference in the frequency of the genotype GA+AA in the AR-E211 genetic polymorphisms between female postadolescent acne patients and healthy control (P=0.028,OR=0.937,OR 95% CI=0.885－0.992);and there was also a significant difference in the allele (A) frequency between female postadolescent acne patients and healthy control (P=－0.029,OR=0.968,OR 95% CI=0.941-0.996).Conclusion The high frequency of A allele in the functional G1733A polymorphism of AR-E211 is a decreased risk factor for female postadolescent acnein Han Chinese.

Key words: androgen receptor;gene polymorphism;female postadolescent acne;han nationality;genetics

中图分类号:

R758.73

田黎明, 谢红付, 杨婷, 王玮蓁, 李吉. 雄激素受体基因 E211 G>A多态性与汉族女性青春期后痤疮的关联性分析[J]. J4, 2011, 37(1): 113-116.

TIAN Li-Ming, XIE Hong-Fu, YANG Ting, WANG Wei-Zhen, LI Ji. Relevance analysis on E211 G>A androgen receptor gene polymorphism and 　 female postadolescent acne in Han population[J]. J4, 2011, 37(1): 113-116.