Tim-3基因4个SNP位点多态性与宁夏地区回族人群

J4 ›› 2011, Vol. 37 ›› Issue (1): 117-121.

Tim-3基因4个SNP位点多态性与宁夏地区回族人群

杨易,徐金瑞,张东涛,孙晶莹

宁夏大学西部特色生物资源保护与利用教育部重点实验室|宁夏银川 750021

收稿日期:2010-06-30 出版日期:2011-01-28 发布日期:2011-01-28
通讯作者: 徐金瑞（Tel：0951-2062813，E-mail:xjr975@163.com） E-mail:xjr975@163.com
作者简介:杨易（1976-）|女|贵州省松桃县人|讲师|遗传学硕士|主要从事遗传学研究
基金资助:
宁夏回族自治区科技厅自然科学基金资助课题（NZ0933）

Correlation between polymorphisms of 4 SNPs of Tim-3 gene and suceptibility of |rheumatoid arthritisin Ningxia Hui population

YANG Yi, XU Jin-Rui, ZHANG Dong-Tao, SUN Jing-Ying

Received:2010-06-30 Online:2011-01-28 Published:2011-01-28

摘要/Abstract

摘要：

目的：探讨宁夏地区回族人群中Tim-3基因4个单核苷酸多态性(SNP)位点多态性与类风湿性关节炎（RA）易感性的关联性，为RA的早期预防提供理论依据。方法：采用序列特异性引物聚合酶链式反应（SSP-PCR）及限制性片段长度多态性（PCR-RFLP）2种方法检测113例RA患者及111例健康个体Tim-3基因-574、-882、-1541和4259共4个位点的单核苷酸多态性。结果：宁夏回族健康人群和RA患者中，Tim-3基因-574、-882、-1541基因型频数和等位基因频数的分布差异均无统计学意义（P>0.05），4259位点基因型和等位基因频数分布差异有统计学意义（P<0.05），RA患者组G等位基因频数（6.36%）显著高于健康组（1.58%）。结论：宁夏回族人群Tim-3基因-574、-882、-1541和4259位点存在单核苷酸多态性变异，其中4259T>G的变异与宁夏回族人群RA发生有关。

关键词: 类风湿性关节炎；Tim-3基因；单核苷酸多态性;回族

Abstract:

Objective
To investigate the correlation between the polymorphisms of 4 SNPs of Tim-3 gene and susceptibility of rheumatoid arthritis (RA) in Ningxia Hui population and provide theory basis for taking precautions against RA. Methods Polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP),specific sequence primer-polymerase chain reaction (SSP-PCR) genotyping method were carried out to examine the SNPs at -574, -882,-1541 and 4259 sites of Tim-3 gene,including 111 healthy individuals and 113 RA patients in Ningxia Hui population. Results Both genotypic frequency and allelic frequency at -574,-882,-1541 sites of Tim-3 gene had no significant difference（P>0.05）,while both genotypic frequency and allelic frequency at 4259 site had significant difference between patient group and normal group（P<0.05）. The frequency of allele G in patient group was significantly higher than that in normal group（P<0.05).Conclusion -574,-882,-1541 and 4259 sites of Tim-3 gene have SNP variations,and 4259 T>G mutation gene is related to the pathogenesis of RA in Ningxia Hui population.

Key words: rheumatoid arthritis;Tim-3 gene;single nucleotide polymorphism;Hui nationality

中图分类号:

R593.22

杨易,徐金瑞,张东涛,孙晶莹. Tim-3基因4个SNP位点多态性与宁夏地区回族人群[J]. J4, 2011, 37(1): 117-121.

YANG Yi, XU Jin-Rui, ZHANG Dong-Tao, SUN Jing-Ying. Correlation between polymorphisms of 4 SNPs of Tim-3 gene and suceptibility of |rheumatoid arthritisin Ningxia Hui population[J]. J4, 2011, 37(1): 117-121.