转录因子Ets1和Ets2在小鼠睾丸组织中的表达及其意义

J4 ›› 2011, Vol. 37 ›› Issue (1): 6-10.

转录因子Ets1和Ets2在小鼠睾丸组织中的表达及其意义

刘洋|金波|郭斌|赵丽红|韩玉帅|岳占碰|张学明

(吉林大学畜牧兽医学院动物胚胎工程吉林省重点实验室|吉林长春 130062)

收稿日期:2010-08-16 出版日期:2011-01-28 发布日期:2011-01-28
通讯作者: 张学明（ E-mail:zhangxuem@jlu.edu.cn
作者简介:刘洋（1985－）|男|黑龙江省大庆市人|基础兽医学硕士|主要从事Ets转录因子对小鼠睾丸组织调控研究。

Expressions of transcription factors Ets1 and Ets2 in mouse testis tissue and significances

LIU Yang, JIN BO, GUO Bin, DIAO Li-Gong, HAN Yu-Shuai, YUE Zhang-Peng, ZHANG Xue-Meng

（Key Laboratory of Animal Embryo Engineering of Jilin Province,College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China）

Received:2010-08-16 Online:2011-01-28 Published:2011-01-28
Supported by:
国家自然科学基金资助课题（30771555）

摘要/Abstract

摘要：

目的：检测Ets家族转录因子Ets1和Ets2在小鼠睾丸组织中的表达，探讨其对小鼠睾丸发育的调控及其对精原干细胞（SSCs）增殖、分化的可能影响。方法：取生后第1、5、10、15、20、25、30、35、40、50和70天的小鼠睾丸组织，对成年小鼠进行白消安10 mg?kg-1腹腔注射，分别在注射后第0、3、5、8、10和18天取睾丸组织，用半定量RT-PCR方法对比内参β-actin在对应组织内的表达水平，分析Ets1、Ets2 mRNA在睾丸组织中的相对表达量。结果：Ets1的表达量在生后第1~30天显著高于出生后第35天（P<0.05或P<0.01），之后明显降低并保持稳定；Ets2在生后第1~25天表达量显著高于第35天（P<0.05或P<0.01），之后明显下降并保持稳定。白消安处理后，Ets1的表达量于第5天降至最低，随后逐渐恢复，第9天后基本达到处理前水平并保持相对稳定，其中第5、8天表达量均显著低于处理后第0天（P<0.05或P<0.01）；Ets2的表达量在白消安处理后前期变化不明显，第10天时明显降低，与第0和18天比较差异有统计学意义（P<0.05），之后缓慢回升，第18天左右恢复至处理前水平。结论：转录因子Ets1和Ets2可能对睾丸早期发育、成年精子发生的维持及精原细胞的增殖、分化具有调节作用。

关键词: Ets转录因子家族；Ets1；Ets2；睾丸；白消安；小鼠

Abstract:

Abstract:Objective To detect the expressions of Ets family transcription factors Ets1 and Ets2 in mouse testis tissue and explore the effects of Ets1 and Ets2 on the development of mouse testis and self-renewal and differentiation of spermatogonial stem cells(SSCs). Methods The mouse testis tissues were collected from specific developmental stages including postnatal days 1,5,10,15,20,25,30,35,40,50 and 70;Busulfan peritoneal injection was performed and mouse testis tissues were collected on the 0th,3rd,5th,8th,10th,18th days after injection, respectively. The mRNA expression levels of Ets1 and Ets2 in samples were analyzed by semi-quantitative RT-PCR with β-actin as the internal control. Results The expression of Ets1 was significantly higher during the period of postnatal 1-30 d than that at postnatal day 35 (P<0.05 or P<0.01),while its expression was decreased evidently and maintained at a stable level afterwards. The expression of Ets2 was significantly higher during the period of postnatal 1-25 d than that at postnatal day 35 (P<0.05 or P<0.01),while it was decreased significantly and maintained at a stable level afterwards similarly. After busulfan treatment,the expression of Ets1 was declined and reached the lowest level at day 5,then was increased gradually and reached the level of day 0 after busulfan treatment and maintained steadily around day 9. Notably,the expressions of Ets1 at day 5 and day 8 were significantly higher than that of day 0 after busulfan treatment (P<0.05 or P<0.01). No obvious changes were observed for the expression of Ets2 during 1-9 d after busulfan treatment,while it was decreased dramatically at day 10,which was significantly lower than those of day 0 and day 18 (P<0.05 or P<0.01). The expression of Ets2 was gradually increased after day 10 and reached its normal level around day 18. Conclusion Ets1 and Ets2 may affect the early development of mouse testis,adult spermatogenesis,as well as the proliferation and differentiation of SSCs.

Key words: Ets family transcription factors;Ets1;Ets2;testis;busulfan;mice

中图分类号:

刘洋|金波|郭斌|赵丽红|韩玉帅|岳占碰|张学明 . 转录因子Ets1和Ets2在小鼠睾丸组织中的表达及其意义[J]. J4, 2011, 37(1): 6-10.

LIU Yang, JIN BO, GUO Bin, DIAO Li-Gong, HAN Yu-Shuai, YUE Zhang-Peng, ZHANG Xue-Meng . Expressions of transcription factors Ets1 and Ets2 in mouse testis tissue and significances[J]. J4, 2011, 37(1): 6-10.

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