CD133免疫磁珠分选脑肿瘤干细胞及其生物学特性

J4 ›› 2011, Vol. 37 ›› Issue (3): 441-444.

CD133免疫磁珠分选脑肿瘤干细胞及其生物学特性

齐玲¹|金宏¹|丁丽娟²|温娜¹|唐泽波¹|狄阳¹|李蕴潜^2

1.吉林医药学院病理学教研室|吉林吉林 132013；2.吉林大学第一医院神经外科|吉林长春 130021

收稿日期:2011-01-18 出版日期:2011-05-28 发布日期:2011-05-28
通讯作者: 李蕴潜（Tel：0431-85933066，E-mail：yunqianli@gmail.com） E-mail:yunqianli@gmail.com
作者简介:齐玲（1974-）|女|吉林省吉林市人|讲师|医学博士|主要从事脑肿瘤干细胞研究。
基金资助:
吉林省科技厅自然科学基金资助课题（202015242）;吉林省教育厅科研基金资助课题（吉教科合字［2011］第281号，吉教科合字［2010］第532号）。

CD133 magnetic sorting and biological characteristics of brain tumor stem cells 

QI Ling¹,JIN Hong¹,DING Li-juan²,WEN Na¹,TANG Ze-bo¹,DI Yang¹,LI Yun-qian²

1.Department of Pathology,Jilin Medical College,Jilin 132013,China;2.Department of Neurosurgery, |First Hospital,Jilin University,Changchun 130021,China

Received:2011-01-18 Online:2011-05-28 Published:2011-05-28

摘要/Abstract

摘要：

目的：从人脑肿瘤组织中分离、培养、纯化和鉴定脑肿瘤干细胞（BTSCs），探讨CD133免疫磁珠分选方法获取BTSCs的可行性及BTSCs的生物学特性。方法：留取人脑胶质母细胞瘤新鲜标本，分离获得脑肿瘤细胞。应用CD133免疫磁珠分选方法纯化BTSCs；流式细胞仪检测分选阳性率神经球计数法分析CD133+/－亚群细胞神经球形成情况；免疫荧光方法检测CD133+/－亚群细胞表面神经干细胞(NSCs)标记物CD133、神经巢蛋(nestin)表达情况；检测CD133+/－亚群细胞经诱导分化后细胞表面分化标记物β-TubulinⅢ、GFAP表达情况。结果：CD133+亚群胞具有干细胞特性，可形成明显的神经球，具有自我更新和明显的增殖能力，并且NSCs标记CD133、nestin表达阳性，经诱导分化后分化标记物β-TubulinⅢ、GFAP表达阳性；CD133－亚群细胞无上述特性。结论：CD133免疫磁珠分选方法获得的CD133+亚群细胞即为BTSCs，该方法可以得到高纯度的BTSCs，可以用于BTSCs的实验研究。

关键词: 脑肿瘤干细胞；神经干细胞；CD133；神经巢蛋白

Abstract:

Abstract:Objective To isolate and culture brain tumor stem cells(BTSCs),and explore the feasibility for obtaining BTSCs byCD133 magnetic sorting and the biological characteristics of BTSCs. Methods The fresh glioblastoma sample wasdissociated,the cells were cultivated in serum-free media with growth factor,the BTSCs were purified by CD133 magneticsorting,the positive rate of CD133+/- subgroup cells was detected by flowcytometry; neurosphere forming essay was used toanalyze the self-renewal and proliferativecapacity; immunofluorescent staining was performed to detect the neural stem cells(NSCs) markers CD133 and nestin of CD133+/- subgroup cells,and the multipotentiality markers β-TubulinⅢ and GFAP ofCD133+/- subgroup cells after induction. Results The CD133+ cells had the characteristics of stem cells,and could form the neurosphere,had the self-renewal and proliferative capacity. The expressions of CD133 and nestin were positive inCD133+cells;β-TubulinⅢ and GFAP were also positive after differentiation. But CD133－cells hadn’t the characteristics mentioned above. Conclusion CD133 magnetic sorting can isolate and purify BTSCs,it can be used to get more BTSCs for experiments.

Key words: brain tumor stem cells;neurastem cells;CD133;nestin

中图分类号:

Q813

齐玲|金宏|丁丽娟|温娜|唐泽波|狄阳|李蕴潜. CD133免疫磁珠分选脑肿瘤干细胞及其生物学特性[J]. J4, 2011, 37(3): 441-444.

QI Ling,JIN Hong,DING Li-juan,WEN Na,TANG Ze-bo,DI Yang,LI Yun-qian. CD133 magnetic sorting and biological characteristics of brain tumor stem cells [J]. J4, 2011, 37(3): 441-444.