重组人乳铁蛋白对Tca8113细胞中促炎性反应细胞因子IL-6和IL-8蛋白表达的影响

J4 ›› 2011, Vol. 37 ›› Issue (5): 805-807.

• 基础研究 • 上一篇下一篇

重组人乳铁蛋白对Tca8113细胞中促炎性反应细胞因子IL-6和IL-8蛋白表达的影响

张振波¹, 张玉英², 王雷²

1. 长春医学高等专科学校口腔学教研室|吉林长春 |130031；2. 吉林大学口腔医院牙周病科,吉林长春 |130021）

收稿日期:2011-06-08 出版日期:2011-09-28 发布日期:2011-09-28
通讯作者: 王雷 E-mail:（Tel:0431-88796039，E-mail:wlandni@yahoo.com.cn）
作者简介:张振波（1972-）|女|吉林省长春市人|讲师|主要从事口腔医学基础及临床研究。
基金资助:
吉林省科技厅科技发展计划项目资助课题（200705346)

Effects of recombinant human lactoferrin on expressions of |pro-inflammatory cytokine IL-6 and IL-8 in Tca8113 cells

ZHANG Zhen-Bo¹, ZHANG Yu-Ying², WANG Lei²

(1. Department of Stomatology, Changchun Medical College,Changchun 130031,China;2.Department of Periodontics, Stomatology Hospital|Jilin University,Changchun |130021,China)

Received:2011-06-08 Online:2011-09-28 Published:2011-09-28

摘要/Abstract

摘要：

目的：研究重组人乳铁蛋白（rhLF）对口腔鳞癌Tca8113细胞中促炎症反应细胞因子IL-6和IL-8表达的影响，探讨其抑制肿瘤细胞生长的可能机制。方法：体外培养口腔鳞癌Tca8113细胞，用rhLF处理体外培养的Tca8113细胞，应用酶联免疫吸附试验、免疫组织化学方法检测50 mg•L^-1rhLF和100 mg•L^-1rhLF对Tca8113细胞分泌的IL-6和IL-8蛋白表达的影响。结果：随着rhLF浓度的增加，培养上清中的IL-6和IL-8含量逐渐下降；100 mg•L-1rhLF组培养上清中IL-6和IL-8水平明显下降，与正常对照组比较差异有统计学意义（P<0.05）。免疫组化染色，IL-6、IL-8在Tca8113细胞有表达，与对照组比较，50 mg•L^-1rhLF组IL-6、IL-8　在Tca8113细胞的表达明显下降，表现为弱表达或不表达。结论：rhLF在体外可以特异性地抑制口腔鳞癌Tca8113细胞中IL-6和IL-8的生成，诱导肿瘤细胞炎症性环境的改变，达到抑制肿瘤生长的目的。

关键词: 重组人乳铁蛋白；口腔鳞状细胞癌；促炎性反应细胞因子；白细胞介素6；白细胞介素8

Abstract:

Abstract:Objective
To study the effects of recombinant human lactoferrin( rhLF ) on the expressions of pro-inflammatory cytokine IL-6 and IL-8 in Tca8113 cells in oral squamous cell carcinoma and to explore the possible mechanism of inhibiting the growth of tumor cells.Methods The Tca8113 cells cultivated in vitro were treated with rhLF(50 and 100 mg•L^-1).Enzyme linked immunosorbent assay and immunohistochemical method were used to detect the influence of rhLF on the expressions of IL-6 and IL-8 proteins.Results The levels of IL-6 and IL-8 in supernatant were gradually decreased with the increasing of the concentrations of rhLF.Compared with normal control group,the levels of IL-6 and IL-8 in 100 mg•L^-1 rhLF group were significantly decreased (P<0.05).The results of immunohistochemical staining showed that the expressions of IL-6 and IL-8 were detected in Tca8113 cells.Compared with control group,the expressions of IL-6 and IL-8 in 50 mg•L^-1rhLF group were significantly decreased,there was only weak expression or no expression in this group.Conclusion rhLF can specificially inhibit the generation of IL-6 and IL-8 in Tca8113 cells and induce the changes of inflammatory environment of tumor cells to inhibit the growth of tumor.

Key words: recombinate human lactoferrin;oral squamous cell carcinoma;pro-inflammatory cytokine;interleukin-6;interleukin-８

中图分类号:

R739.8

张振波, 张玉英, 王雷. 重组人乳铁蛋白对Tca8113细胞中促炎性反应细胞因子IL-6和IL-8蛋白表达的影响[J]. J4, 2011, 37(5): 805-807.

ZHANG Zhen-Bo, ZHANG Yu-Ying, WANG Lei. Effects of recombinant human lactoferrin on expressions of |pro-inflammatory cytokine IL-6 and IL-8 in Tca8113 cells[J]. J4, 2011, 37(5): 805-807.

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