特定寡核苷酸对大鼠实验性牙移动的影响

J4 ›› 2011, Vol. 37 ›› Issue (5): 812-816.

特定寡核苷酸对大鼠实验性牙移动的影响

王静^1,2, 孙新华¹, 冯志远¹, 申玉芹³

1.吉林大学口腔医院正畸科|吉林长春130021；2.河北省沧州市人民医院正畸科|河北沧州 061000； 3.吉林大学口腔医院牙周病科|吉林长春130021）

收稿日期:2011-04-18 出版日期:2011-09-28 发布日期:2011-09-28
通讯作者: 孙新华 E-mail:(Tel : 0431-88796023,E-mail : xinhuasun8@163.com)
作者简介:王静 (1985-),女,河北省沧州市人,口腔医学硕士,主要从事牙齿移动生物学的研究。
基金资助:
吉林省科技厅自然科学基金资助课题（201015203）

Influence of specific oligodeoxynucleotides on |experimental tooth movement in rats

WANG Jing^1,2, SUN Xin-Hua¹, FENG Zhi-Yuan¹, SHEN Yu-Qin³

(1. Department of Orthodontics,Stomatology Hospital,Jilin University,Changchun 130021,China;2. Department of Orthodontics,People’s Hospital of Cangzhou City, Hebei Province,Cangzhou 061000,China;3. Department of Peridontics, Stomatology Hospital|Jilin University,Changchun 130021,China)

Received:2011-04-18 Online:2011-09-28 Published:2011-09-28

摘要/Abstract

摘要：

目的：探讨特定寡核苷酸（ODN）对大鼠实验性牙移动的影响，阐明其在牙周组织改建中的作用特点。方法：40只雄性Wistar大鼠，随机分为实验组(n=20)和对照组（n=20），建立大鼠牙移动模型。加力当天于实验组大鼠两侧上颌第一磨牙颊侧牙龈黏膜下各注射50 μL 特定ODN（20 mg•L^-1），对照组则于相同部位注射等量的PBS溶液，每3 d给药1次，取3、7、14、21和28 d 5个时间点各处死4只大鼠。建模前和处死前取模型测定牙移动距离，经HE染色观察牙周组织变化并计数破骨细胞(OC)。结果：大鼠牙移动距离随时间变化逐渐增大，除3 d组外实验组大鼠第一磨牙牙移动距离均小于对照组（P<0.05或P<0.01）；HE染色观察，实验组大鼠第一磨牙压力侧牙槽骨吸收程度轻于对照组，OC数少于对照组， 3、7 和14 d时2组间差异具有统计学意义（P<0.05或P<0.01）。结论：特定ODN可减少压力侧牙槽骨表面OC的数量从而抑制实验性牙齿移动，对正畸牙周组织的改建产生影响。

关键词: 寡核苷酸；正畸；牙移动；牙周组织

Abstract:

Abstract:Objective
To study the influence of specific oligodeoxynucleotides (ODN) on the experimental tooth movement in rats and to clarify its role in the reconstruction of periodontal tissues. Methods Fourty male Wistar rats were randomly divided into experiment group (n= 20) and control group (n= 20).The tooth movement models were set up.Then 50μL specific ODN (20 mg•L^-1) was injected into both sides of the buccal gingival mucosa adjacent to the first upper molar in experiment group,the rats in control group were injected by the same amount of PBS in the sam place,once every three days.4 rats were killed after 3,7,14,21 and 28 d in each group.Then the distance of tooth movement was measured before the models were made and before the rats was killed,and the changes of periodontal tissues were observed by HE staining and the number of osteoclasts was counted. Results The distance of tooth movement in rats were gradually increased. Except for 3 d group,the distances in experiment group were shorter than those in control group（P<0.05 or P<0.01）. HE staining showed that the absorption on pressure side of the first molar alveolar bone and the number of osteoclasts in experiment group were less than those in control group, especially on the days of 3,7 and 14（P<0.05 or P<0.01）.Conclusion The specific ODN can inhibit the tooth movement by reducing the number of OC on the pressure side of alveolar bone,so the specific ODN can influence the reconstruction of periodontal tissues.

Key words: oligonucleotides;orthodontics;tooth movement;periodontal tissue

中图分类号:

R783.5

王静, 孙新华, 冯志远, 申玉芹. 特定寡核苷酸对大鼠实验性牙移动的影响[J]. J4, 2011, 37(5): 812-816.

WANG Jing, SUN Xin-Hua, FENG Zhi-Yuan, SHEN Yu-Qin. Influence of specific oligodeoxynucleotides on |experimental tooth movement in rats[J]. J4, 2011, 37(5): 812-816.