中国北方汉族人群HLA-DRB1单核苷酸多态性与脑血管病的关系

J4 ›› 2011, Vol. 37 ›› Issue (5): 867-872.

中国北方汉族人群HLA-DRB1单核苷酸多态性与脑血管病的关系

杨雪玲¹|丁艳华²|李青梅³|徐珊珊⁴|徐秀芝⁵|杨思嘉⁴|张雪岩*|李波⁴|刘娅¹

1. 吉林大学公共卫生学院营养与食品卫生学教研室|吉林长春|130021；2. 吉林大学第一医院Ⅰ期药物临床实验病房|吉林长春 130021；3. 吉林大学第一医院儿科|吉林长春 130021；4. 吉林大学公共卫生学院流行病与卫生统计学教研室|吉林长春 130021；5.长春卫尔赛生物药业有限公司|吉林　长春　130616

收稿日期:2011-05-24 出版日期:2011-09-28 发布日期:2011-09-28
通讯作者: 李波（Tel：0431-85619451， E-mail：li_bo@jlu.edu.cn）；刘娅（Tel：0431-85166352， E-mail：liuya@jlu.edu.cn） E-mail:li_bo@jlu.edu.cn；liuya@jlu.edu.cn
作者简介:杨雪玲(1985-)|女|河南省扶沟县人|在读医学博士|主要从事流行病学和卫生毒理学方面的研究。
基金资助:
国家自然科学基金资助课题（30872174）

Association between HLA-DRB1 SNPs |and cerebrovascular disease in Han population from North China

YANG Xue-ling¹,DING Yan-hua²,LI Qing-mei³,XU Shan-shan⁴,XU Xiu-zhi⁵,YANG Si-jia⁴,ZHANG Xue-yan*,LI Bo⁴,LIU Ya¹

1. Department of Nutrition and Food Hygiene,School of Public Health,Jilin University,Changchun 130021,China;2. Ward of PhaseⅠClinical Drug Trials,First Hospital,Jilin University,Changchun 130021,China;3. Department of Pediatrics,First Hospital,Jilin University,Changchun 130021,China|4. Department of Epidemiology and Statistics,School of Public Health,Jilin University,Changchun 130021,China;5.Changchun Weresai Biotech Pharmaceutical Co.,LTD,Changchun 130616,China

Received:2011-05-24 Online:2011-09-28 Published:2011-09-28

摘要/Abstract

摘要：

目的：研究北方汉族人群脑血管病与HLA-DRB1单核苷酸多态性的遗传关联性，为脑血管病的免疫遗传学研究奠定基础。方法：采用病例对照研究方法，收集160 例脑血管病患者和139例健康对照。根据知情同意原则对每例受检者进行身高、体质量、腰围（WC）、腹围（AC）、体质量指数（BMI）等一般指标测量，采集空腹静脉血，检测血白蛋白（ALB）、血红蛋白（Hb）、血肌酐（Cr）、血尿酸（UA）、空腹血糖（FBG）、总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白（HDL）和低密度脂蛋白（LDL）等生化指标，并提取基因组DNA。应用聚合酶链式反应-连接酶检测反应（PCR-LDR）技术检测HLA-DRB1基因上rs2308765、rs3135388及rs9269186位点的单核苷酸多态性。采用拟合优度χ2检验分析基因型分布是否符合Hardy-Weinberg平衡，应用χ2检验分析病例组与对照组基因型和等位基因频数分布。结果：病例组和对照组WC、ALB、Hb、FBG、TC和TG比较差异无统计学意义（P>0.05）；病例组BMI、AC、Cr和UA均高于对照组(P<0.05)，HDL低于对照组（P<0.05）；病例组和对照组rs2308765、rs3135388及rs9269186的基因型频数分布和等位基因频数分布差异均无统计学意义（P>0.05）。结论：北方汉族人群脑血管病的发生可能与HLA-DRB1的rs2308765、rs3135388及rs9269186单核苷酸多态性无关联。

关键词: 脑血管病；HLA-DRB1；单核苷酸多态性

Abstract:

Abstract:Objective To evaluate the association between HLA-DRB1 single nucleotide polymorphisms (SNPs) and cerebrovascular disease in the Han population from North China,and to lay a foundation for immunogenetic research on cerebrovascular disease.Methods 160 patients with cerebrovascular disease and 116 controls were enrolled in the case-control study.According to the principle of informed consent,the indexes of height,weight,waist circumference (WC),abdominal circumference (AC) and body mass index (BMI) were measured;and the venous blood was drawn for the analysis of albumin (ALB),hemoglobin (Hb),blood creatinine (Cr),blood uric acid (UA),fasting plasma glucose (FBG),total cholesterol (TC),triglycerides (TG),high-density lipoprotein (HDL) and low-density lipoprotein (LDL) and DNA extraction.The SNPs of rs2308765,rs3135388 and rs9269186 in HLA-DRB1 were detected by PCR-LDR assay.The goodness-of-fit χ2 test was used for Hardy-Weinberg equilibrium test in case and control groups.The differences of genotypic frequency and allelic frequency between case and control were test by χ2 test.Results There were no significant differences of WC,ALB,Hb,FBG,TC and TG between two groups (P>0.05);Compared with control group,the BMI,AC,Cr and UA in case group were higher than that in control group (P<0.05),while HDL was lower (P<0.05).There were no significant differences of distributions of genotypes and alleles of rs2308765,rs3135388 and rs9269186 sites between two groups.Conclusion There is not a certain correlation between rs2308765,rs3135388,and rs9269186 SNPs and cerebrovascular disease in Han population from North China.

Key words: cerebrovascular disease;human leukocyte antigen DR beta-1;single nucleotide polymorphism

中图分类号:

R743.3

杨雪玲|丁艳华|李青梅|徐珊珊|徐秀芝|杨思嘉|张雪岩|李波|刘娅. 中国北方汉族人群HLA-DRB1单核苷酸多态性与脑血管病的关系[J]. J4, 2011, 37(5): 867-872.

YANG Xue-ling,DING Yan-hua,LI Qing-mei,XU Shan-shan,XU Xiu-zhi,YANG Si-jia. Association between HLA-DRB1 SNPs |and cerebrovascular disease in Han population from North China[J]. J4, 2011, 37(5): 867-872.

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