辐射诱导表达载体pshuttle-Egr1-shTRAIL对肝癌SMMC7721细胞生长的抑制作用

J4 ›› 2011, Vol. 37 ›› Issue (6): 981-984.

辐射诱导表达载体pshuttle-Egr1-shTRAIL对肝癌SMMC7721细胞生长的抑制作用

陈志勇^1,2, 刘敏², 龚守良³, 董丽华²

(1. 内蒙古民族大学附属医院肿瘤科|内蒙古通辽 028000;2. 吉林大学第一医院肿瘤中心放疗科|吉林长春130021;3.吉林大学公共卫生学院卫生部放射生物学重点实验室|吉林长春130021)

收稿日期:2011-06-08 出版日期:2011-11-28 发布日期:2011-11-28
通讯作者: 董丽华 E-mail:（Tel: 0431-88782468,E-mail: dlh2003cn2003@yahoo.com.cn）
作者简介:陈志勇（1974-）,男,内蒙古自治区通辽市人,主治医师|医学硕士,主要从事肿瘤放射治疗方面的研究。
基金资助:
国家自然科学基金资助课题（30570546）

Inhibitory effects of recombinant plasmid pshuttle-Egr1-shTRAIL transfection in combination with X-irradiation on growth of liver cancer cells SMMC7721

CHEN Zhi-Yong^1,2, LIU Min², GONG Shou-Liang³, DONG Li-Hua²

（1. Department of Oncology,Affiliated Hospital of Innermongol Nation University,Tongliao 028000,China；2. Department of Radiotherapy,Tumor Center,First Hospital,Jilin University,Changchun130021,China；3. Key Laboratory of Radiobiology,Ministry of Health,School of Public Health,Jilin University,Changchun 130021,China）

Received:2011-06-08 Online:2011-11-28 Published:2011-11-28

摘要/Abstract

摘要：

目的：研究携带可溶性人肿瘤坏死因子相关凋亡诱导配体(shTRAIL)基因的辐射诱导表达载体pshuttle-Egr1-shTRAIL在人肝癌细胞株中的表达及其促凋亡作用。方法：体外通过脂质体转染SMMC7721细胞。转染细胞分别接受0(假照组)、2、5和10 Gy X线照射，ELISA法检测sTRAIL的表达；细胞分为正常SMMC7721组，分别接受0(假照组)、2和5 Gy X线照射的转染空质粒pshuttle-Egr1组及转染重组质粒pshutlle-Eyr1-shTRAIL组，采用AnnexinV-FITC凋亡检测试剂盒检测细胞凋亡率；分别取SMMC772细胞、转染STRAIL细胞、转染空载体及照射组细胞，采用克隆形成实验检测细胞存活率。结果：不同剂量X射线照射SMMC7721-shTRAIL细胞上清中可溶性TRAIL表达量明显高于假照组（P< 0.001）；与假照组比较，照射转染后SMMC7721细胞的凋亡细胞百分数明显增加（P<0.05或P<0.001），细胞存活率明显下降（P<0.05
或P<0.001）。结论：pshuttle-Egr1-shTRAIL

Abstract:

Abstract:Objective To investigate the effects of recombinant plasmid pshuttle-Egr1-shTRAIL stable transfection in combination with X-ray irradiation on the TRAIL protein expression and the apoptosis in human SMMC7721 hepatoma cells. Methods The pshuttle-Egr1-shTRAIL packaged with liposome was stably transfected into SMMC7721 cells in vitro.The shTRAIL protein expressions were measured with ELISA assay,Annexin V-FITC kit was adopted to measure the apoptosis of pshuttle-Egr1-shTRAIL cells,and the changes in survival rate of SMMC7721 cells were measured　with cell cloning assay.Results The TRAIL protein expressions in pshuttle-Egr1-shTRAIL plus different doses of irradiation groups were significantly increased compared with 0 Gy group（P< 0.001）.The percentage of apoptotic cells was significantly higher than that in 0 Gy group (P<0.05 or P<0.001),and the survival rate of SMMC7721 cells was decreased significantly (P<0.05 or P<0.001).Conclusion The pshuttle-Egr1-shTRAIL stable transfection in combination with irradiation can significantly induce the apoptosis of SMMC7721 tumor cells and inhibit the cell proliferation.

Key words: pshuttle-Egr1-shTRAIL;gene clone;human hepatoma SMMC7721;irradiation;gene therapy

中图分类号:

R735.7

陈志勇, 刘敏, 龚守良, 董丽华. 辐射诱导表达载体pshuttle-Egr1-shTRAIL对肝癌SMMC7721细胞生长的抑制作用[J]. J4, 2011, 37(6): 981-984.

CHEN Zhi-Yong, LIU Min, GONG Shou-Liang, DONG Li-Hua. Inhibitory effects of recombinant plasmid pshuttle-Egr1-shTRAIL transfection in combination with X-irradiation on growth of liver cancer cells SMMC7721[J]. J4, 2011, 37(6): 981-984.