卵巢浆液性囊腺癌组织中骨桥蛋白的表达及其临床意义

J4 ›› 2012, Vol. 38 ›› Issue (1): 123-126.

卵巢浆液性囊腺癌组织中骨桥蛋白的表达及其临床意义

李艳艳¹|张松灵¹|张晓霞¹|周恒¹|王医术²

1.吉林大学第一医院妇产科|吉林长春 130021；2.吉林大学白求恩医学院病理解剖学教研室病理生物学教育部重点实验室| 吉林长春 130021

收稿日期:2011-11-27 出版日期:2012-01-28 发布日期:2012-01-28
通讯作者: 张晓霞（Tel:0431-88782182，E-mail:zhangxx626@yahoo.com.cn） E-mail:zhangxx626@yahoo.com.cn
作者简介:李艳艳（1985-）|女|吉林省四平市人|在读医学硕士|主要从事卵巢癌诊断的研究。
基金资助:
国家自然科学基金青年基金资助课题(30901589)

Expression of osteopontin in ovarian serous cystadenocarcinoma tissues and its clinical significance

LI Yan-yan¹,ZHANG Song-ling¹,ZHANG Xiao-xia¹,ZHOU Heng¹,WANG Yi-shu²

1. Department of Obstetrics and Gynecology,First Hospital,Jilin University,Changchun 130021,
China|2. Key Laboratory of Pathobiology,Ministry of Education,Department of Pathologic Anatomy,
Norman Bethune College of Medicine,Jilin University,Changchun 130021,China

Received:2011-11-27 Online:2012-01-28 Published:2012-01-28

摘要/Abstract

摘要：

目的：研究骨桥蛋白(OPN)在卵巢浆液性囊腺癌组织中的表达，探讨其临床意义。方法：　采用免疫组织化学方法检测70例卵巢浆液性囊腺癌组织、22例卵巢浆液性囊腺瘤组织和15例正常卵巢组织中OPN的表达率。结果：① OPN在卵巢浆液性囊腺癌实质细胞中的阳性表达率（82.9%）显著高于卵巢浆液性囊腺瘤组织（22.7%）和正常卵巢组织（13.3%），差异有统计学意义(P<0.05)。② OPN在Ⅰ期卵巢浆液性囊腺癌实质细胞中的阳性表达率为60.0％，Ⅱ期为76.2％，Ⅲ期为96.2％，Ⅳ期为100.0％，Ⅲ期和Ⅳ期分别与Ⅰ期比较差异有统计学意义（P<0.05）；OPN在晚期（Ⅲ-Ⅳ期）卵巢浆液性囊腺癌实质细胞中的阳性表达率为97.1％，早期（Ⅰ-Ⅱ期）为69.4％，两者比较差异有统计学意义(P<0.05)。OPN在高分化（G1）卵巢浆液性囊腺癌实质细胞中阳性表达率为74.1％，中分化（G2）为.0％，低分化（G3）为95.7％，G3组与G1组比较差异有统计学意义（P<0.05），OPN在卵巢浆液性囊腺癌组织中的表达与临床分期和病理分级有关，卵巢浆液性囊腺癌分期越晚、分化越差，OPN阳性表达率越高。③合并有腹水的卵巢浆液性囊腺癌组织的阳性表达率高于无腹水（P<0.05）。结论：OPN促进卵巢浆液性囊腺癌的发生、发展、浸润及转移,OPN表达检测可作为判定卵巢浆液性腺癌的恶性程度和预测预后的指标之一。

关键词: 骨桥蛋白；卵巢浆液性囊腺肿瘤；免疫组织化学

Abstract:

Objective To investigate the expression of osteopontin(OPN) in ova
rian serous cystadenocarcinoma tissues and explore the relationship with clinicopathological characteristics.
Methods The expressions of OPN in 70 cases of ovarian serous cystadenocarcinoma tissues,22 cases of ovarian serous cystadenoma tissues,and 15 cases of normal ovarian tissues were detected by immunohistochemistry method.Results ① The positive expression rate of OPN in ovarian serous cystadenocarcinoma tissues was 82.9%,much higher than those in ovarian serous cystadenoma tissues(22.7%) and nomal ovarian tissues(13.3%)(P<0.05).② The positive expression rates of OPN in stage Ⅰ，stage Ⅱ，stage Ⅲ，stage Ⅳovarian serous cystadenocarcinoma tumor tissues were 60.0％, 76.2％, 96.2％,and 100.0％.The po
sitive rate of OPN expression in stageⅢ and Ⅳ tumor tissues(97.1%) was dramatically higher than that in stage Ⅰand stage Ⅱ (69.4%)(P<0.05).The positive expression rates of OPN in G1，G2，and G3 ovarian serous cystadenocarcinoma tumor tissues were 74.1％,80.0％ and 95.7％,it was dramatically higher in G3 tumor tissues than that in G1 tumor tissues(P<0.05).There was correlation between the positive rate of OPN
and pathological grading and clinical stages,the later clinical stage and the worse differentiation of ovarian serous cystadenocarcinoma,the stronger positive expression rate of OPN.③The positive expression rate of OPN in ovarian serous cystadenocarcinoma tumor tissues with ascites（95.2%）was higher than that in tumor tissues without ascites(64.3%)(P<0.05).Conclusion Overexpression of OPN may promote the development,invasion and metastasis of ovarian serous cystadenocarcinom.The detection of OPN can be used as a prognostic marker in judgement of malignant degree and prognosis in ovarian cancer patients.

Key words: osteopontin, ovarian serous cystadenocarcinoma, immunohistochemistry

中图分类号:

R737.31

李艳艳|张松灵|张晓霞|周恒|王医术. 卵巢浆液性囊腺癌组织中骨桥蛋白的表达及其临床意义[J]. J4, 2012, 38(1): 123-126.

LI Yan-yan,ZHANG Song-ling,ZHANG Xiao-xia,ZHOU Heng,WANG Yi-shu. Expression of osteopontin in ovarian serous cystadenocarcinoma tissues and its clinical significance[J]. J4, 2012, 38(1): 123-126.

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