低剂量氢醌对大鼠骨髓间充质干细胞生物学性状及PARP-1表达的影响

J4 ›› 2012, Vol. 38 ›› Issue (2): 216-220.

低剂量氢醌对大鼠骨髓间充质干细胞生物学性状及PARP-1表达的影响

高羽亭^1,2|刘林华²|黄明元²|梁海荣³|范洪学¹|唐焕文²

（1.吉林大学公共卫生学院卫生毒理学教研室|吉林长春 130021；2.广东医学院公共卫生学院劳动卫生与环境卫生教研室|广东东莞 523808；3.广东医学院公共卫生学院实验中心|广东东莞 523808）

收稿日期:2011-12-30 出版日期:2012-03-28 发布日期:2012-03-28
通讯作者: 唐焕文 E-mail:（Tel:0769-22896569，E-mail: gdmcthw@126.com）
作者简介:高羽亭（1982-）|女|安徽省安庆市人|讲师|在读医学博士|主要从事毒理学方面的研究。
基金资助:
国家自然科学基金资助课题（30972500）；广东省科技厅自然科学基金资助课题（7301507）；广东省湛江市科技局科技攻关计划资助课题（2011C3104004）

Influences of low level hydroquinone in biological characteristics and expression of poly（ADP-ribose）polymerase-1 gene in rat bone mesenchymal stem cells

GAO Yu-ting^1,2,LIU Lin-hua²,HUANG Ming-yuan²,LIANG Hai-rong³,FAN Hong-xue¹,TANG Huan-wen²

(1.Department of Hygienic Toxicology,School of Public Health,Jilin University,Changchun 130021,China;2.Department of Labor Hygiene and Environmental Hygiene,School of Public Health,Guangdong Medical College,Dongguan 532808,China;3.Experimental Center,School of Public Health,Guangdong Medical College,Dongguan 532808,China）

Received:2011-12-30 Online:2012-03-28 Published:2012-03-28

摘要/Abstract

摘要：

目的：探讨低剂量氢醌(HQ)对大鼠骨髓间充质干细胞（BMSCs）生物学性状及聚腺苷二磷酸核糖聚合酶-1(PARP-1)基因表达的影响，阐明低剂量HQ对骨髓的毒效应。方法：磷酸盐缓冲液(PBS)溶解HQ，以PBS处理组为对照组，分别以2.5、5.0和10.0 μmol•L^-1 HQ染毒大鼠BMSCs为处理组。应用Cell Counting Kit-8试剂盒检测细胞活力和细胞增殖能力，通过磷脂结合蛋白(Annexin V) /碘化丙啶(PI)标记的流式细胞技术检测细胞凋亡，用实时荧光定量-聚合酶链反应(qRT-PCR)检测PARP-1基因表达的改变。结果：与PBS对照组比较，各剂量组细胞增殖能力明显提高(P<0.05)；染毒48 h后，5.0和10.0 μmol•L^-1 HQ组细胞的凋亡率均明显降低(P<0.05)；染毒48 h后，2.5、5.0和10.0 μmol•L^-1 HQ组细胞PARP-1表达量分别为对照组0.92、0.56(P<0.05)和0.45倍(P<0.05)。结论：低剂量HQ能抑制大鼠BMSCs凋亡和PARP-1基因表达，促进细胞增殖。

关键词: 氢醌；骨髓间充质干细胞；聚腺苷二磷酸核糖聚合酶-1；细胞凋亡；细胞增殖；大鼠

Abstract:

To explore the influences of low level hydroquinone (HQ) in biological characteristics and the expression of poly(ADP-ribose) polymerase-1(PARP-1)gene in rat bone mesenchymal stem cells(BMSCs),and elucidate the toxic effect of low dose HQ on bone marrow.Methods HQ dissolved in PBS buffer at the concentrations of 2.5,5.0 and 10.0 μmol•L^-1 were respectively given to BMSCs,and the cells treated with PBS only were used as control group.The cell viability and proliferation were detected with CCK-8 assay kit,the apoptosis of BMSCs was measured by Annexin V/PI apoptosis assay kit,while the PARP-1 gene expression was determined by reverse transcription real-time RT-PCR.Results Compared with PBS control group,the ability of cell proliferation raised obviously (P<0.05);the apoptotic rates of BMSCs in 5.0 and 10.0 μmol•L^-1 HQ groups 48 h after exposure were significantly decreased (P<0.05).The expressions of PARP-1 in 2.5,5.0,and 10.0 μmol•L^-1　HQ groups 48　h after exposure were 0.92,0.56(P<0.05),and 0.45 folds of control group(P<0.05).Conclusion Low level HQ could inhibit the apoptosis of BMSCs and PARP-1 gene expression,while promote the cell growth.

Key words: hydroquinone;bone mesenchymal stem cells;poly（ADP-ribose）polymerase-1;apoptosis;cell proliferation;rats

中图分类号:

R329

高羽亭|刘林华|黄明元|梁海荣|范洪学|唐焕文. 低剂量氢醌对大鼠骨髓间充质干细胞生物学性状及PARP-1表达的影响[J]. J4, 2012, 38(2): 216-220.

GAO Yu-ting,LIU Lin-hua,HUANG Ming-yuan,LIANG Hai-rong,FAN Hong-xue,TANG Huan-we. Influences of low level hydroquinone in biological characteristics and expression of poly（ADP-ribose）polymerase-1 gene in rat bone mesenchymal stem cells[J]. J4, 2012, 38(2): 216-220.